Key features and details
- Mouse polyclonal to FAN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAN1 antibody
See all FAN1 primary antibodies
DescriptionMouse polyclonal to FAN1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length human MTMR15 protein (AAH47882, 1 a.a. ~ 533 a.a)
- Human spleen lysate. MTMR15 transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).|
FunctionNuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
Sequence similaritiesBelongs to the FAN1 family.
Contains 1 UBZ-type zinc finger.
Contains 1 VRR-NUC domain.
DomainThe UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
Cellular localizationNucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
- Information by UniProt
- 6030441H18Rik antibody
- Coiled coil domain containing protein MTMR15 antibody
- DKFZp451H236 antibody
Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution
Lane 1 : MTMR15 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab68572 has been referenced in 1 publication.
- Wang Z et al. Microarray gene profiling analysis of glioblastoma cell line U87 reveals suppression of the FANCD2/Fanconi anemia pathway by the combination of Y15 and temozolomide. Arch Med Sci 15:1035-1046 (2019). PubMed: 31360198