Overview

  • Product name

    Anti-FANCA/FAA antibody [EPR16519-42]
    See all FANCA/FAA primary antibodies
  • Description

    Rabbit monoclonal [EPR16519-42] to FANCA/FAA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FANCA/FAA aa 1-150. The exact sequence is proprietary.
    Database link: O15360

  • Positive control

    • WB: HeLa, 293, Jurkat and A431 whole cell lysates; Human colon and fetal kidney lysates. ICC/IF: HeLa and Jurkat cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

     

     

     This product was previously labelled as FANCA

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16519-42
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).

Target

  • Function

    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • Involvement in disease

    Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
  • Cellular localization

    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • FA 1 antibody
    • FA antibody
    • FA H antibody
    • FA1 antibody
    • FAA antibody
    • FACA antibody
    • FAH antibody
    • Fanca antibody
    • FANCA_HUMAN antibody
    • FANCH antibody
    • Fanconi anemia complementation group A antibody
    • Fanconi anemia complementation group H antibody
    • Fanconi anemia group A protein antibody
    • Fanconi anemia type 1 antibody
    • MGC75158 antibody
    • Protein FACA antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: ProteinX knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab201458 observed at 163 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab201458 was shown to recognize FANCA/FAA when FANCA/FAA knockout samples were used, along with additional cross-reactive bands. Wild-type and FANCA/FAA knockout samples were subjected to SDS-PAGE. ab201458 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-FANCA/FAA antibody [EPR16519-42] (ab201458) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (Human embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-FANCA/FAA antibody [EPR16519-42] (ab201458) at 1/1000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 3 minutes

References

ab201458 has not yet been referenced specifically in any publications.

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