Overview

  • Product name

    Anti-FANCA/FAA antibody [EPR16519]
    See all FANCA/FAA primary antibodies
  • Description

    Rabbit monoclonal [EPR16519] to FANCA/FAA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FANCA/FAA aa 1-150. The exact sequence is proprietary.
    Database link: O15360

  • Positive control

    • WB: HeLa, HEK-293, A431 and Jurkat whole cell lysates; Human colon lysate. IP: HeLa whole cell lysate.
  • General notes

     

     

     This product was previously labelled as FANCA

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16519
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).

Target

  • Function

    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • Involvement in disease

    Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
  • Cellular localization

    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • FA 1 antibody
    • FA antibody
    • FA H antibody
    • FA1 antibody
    • FAA antibody
    • FACA antibody
    • FAH antibody
    • Fanca antibody
    • FANCA_HUMAN antibody
    • FANCH antibody
    • Fanconi anemia complementation group A antibody
    • Fanconi anemia complementation group H antibody
    • Fanconi anemia group A protein antibody
    • Fanconi anemia type 1 antibody
    • MGC75158 antibody
    • Protein FACA antibody
    see all

Images

  • Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: FANCA/FAA beta knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab201457 observed at 163 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab201457 was shown to specifically react with FANCA/FAA beta when FANCA/FAA beta knockout samples were used. Wild-type and FANCA/FAA beta knockout samples were subjected to SDS-PAGE. ab201457 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution + Human colon lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201457 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201457 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201457 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

This product has been referenced in:

  • Zhong J  et al. Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress. Int J Biol Sci 13:923-934 (2017). Read more (PubMed: 28808424) »
See 1 Publication for this product

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