Recombinant Anti-FANCB antibody [EPR15513] (ab186729)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15513] to FANCB
- Suitable for: Flow Cyt (Intra), IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FANCB antibody [EPR15513]
See all FANCB primary antibodies -
Description
Rabbit monoclonal [EPR15513] to FANCB -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A549, 293T, U87-MG, C6, Raw264.7 and PC12 cell lysates; Human endometrial adenocarcinoma tissue; 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15513 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/120.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30 - 1/50.
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WB |
1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
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Notes |
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Flow Cyt (Intra)
1/120. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30 - 1/50. |
WB
1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
Target
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Function
DNA repair protein required for FANCD2 ubiquitination. -
Involvement in disease
Defects in FANCB are the cause of Fanconi anemia complementation group B (FANCB) [MIM:300514]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
Defects in FANCB are the cause of X-linked VACTERL-H (XVACTERL-H) [MIM:314390]; also known as X-linked VACTERL association with hydrocephalus syndrome. VACTERL is an acronym for vertebral anomalies, anal atresia, cardiac malformations, tracheoesophageal fistula, renal anomalies (urethral atresia with hydronephrosis), and limb anomalies (hexadactyly, humeral hypoplasia, radial aplasia, and proximally placed thumb). Some cases of VACTERL-H are associated with increased chromosome breakage and rearrangement. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2187 Human
- Entrez Gene: 237211 Mouse
- Entrez Gene: 501552 Rat
- Omim: 300515 Human
- SwissProt: Q8NB91 Human
- SwissProt: Q5XJY6 Mouse
- Unigene: 554740 Human
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Alternative names
- FA2 antibody
- FAAP90 antibody
- FAAP95 antibody
see all
Images
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All lanes : Anti-FANCB antibody [EPR15513] (ab186729) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa -
All lanes : Anti-FANCB antibody [EPR15513] (ab186729)
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 98 kDa
Observed band size: 98 kDa -
Western blot analysis of FANCB in 293T cell lysate immunoprecipitated with ab186729 at 1/50 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling FANCB with ab186729 at 1/500 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed293 cells labeling FANCB with ab186729 at 1/120 dilution, compared to a Rabbit monclonal IgG isotype control. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186729 has been referenced in 1 publication.
- Kato Y et al. FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis. Hum Mol Genet 24:5234-49 (2015). PubMed: 26123487