Recombinant
RabMAb

Anti-FANCB antibody [EPR15513] (ab186729)

Overview

  • Product name
    Anti-FANCB antibody [EPR15513]
  • Description
    Rabbit monoclonal [EPR15513] to FANCB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FANCB aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8NB91

  • Positive control
    • HeLa, A549, 293T, U87-MG, C6, Raw264.7 and PC12 cell lysates; Human endometrial adenocarcinoma tissue; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15513
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/30 - 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).

Target

  • Function
    DNA repair protein required for FANCD2 ubiquitination.
  • Involvement in disease
    Defects in FANCB are the cause of Fanconi anemia complementation group B (FANCB) [MIM:300514]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in FANCB are the cause of X-linked VACTERL-H (XVACTERL-H) [MIM:314390]; also known as X-linked VACTERL association with hydrocephalus syndrome. VACTERL is an acronym for vertebral anomalies, anal atresia, cardiac malformations, tracheoesophageal fistula, renal anomalies (urethral atresia with hydronephrosis), and limb anomalies (hexadactyly, humeral hypoplasia, radial aplasia, and proximally placed thumb). Some cases of VACTERL-H are associated with increased chromosome breakage and rearrangement.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FA2 antibody
    • FAAP90 antibody
    • FAAP95 antibody
    • FAB antibody
    • FACB antibody
    • Fancb antibody
    • FANCB_HUMAN antibody
    • Fanconi anemia associated polypeptide of 95 kDa antibody
    • Fanconi anemia group B protein antibody
    • Fanconi anemia, complementation group B antibody
    • Fanconi anemia-associated polypeptide of 95 kDa antibody
    • Protein FACB antibody
    see all

Images

  • All lanes : Anti-FANCB antibody [EPR15513] (ab186729) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • All lanes : Anti-FANCB antibody [EPR15513] (ab186729)

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • Western blot analysis of FANCB in 293T cell lysate immunoprecipitated with ab186729 at 1/50 dilution

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling FANCB with ab186729 at 1/500 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counter stained with Hematoxylin.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling FANCB with ab186729 at 1/120 dilution, compared to a Rabbit monclonal IgG isotype control. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody.

References

This product has been referenced in:
  • Kato Y  et al. FANCB is essential in the male germline and regulates H3K9 methylation on the sex chromosomes during meiosis. Hum Mol Genet 24:5234-49 (2015). Read more (PubMed: 26123487) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 03 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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