- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-FANCC antibody
See all FANCC primary antibodies -
DescriptionRabbit polyclonal to FANCC
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Human
-
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 275 of FANCC (Q00597).
-
Positive control
- WB: H1299, NIH 3T3, 293T, A431, HepG2 or Raji cell lysate; ICC/IF: HeLa cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 63 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
-
FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
-
Tissue specificityUbiquitous.
-
Involvement in diseaseDefects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
-
Developmental stageExpression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
-
Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
- Information by UniProt
-
Database links
- Entrez Gene: 2176 Human
- Entrez Gene: 14088 Mouse
- Omim: 613899 Human
- SwissProt: Q00597 Human
- SwissProt: P50652 Mouse
- Unigene: 494529 Human
-
Alternative names
- bA80I15.1 antibody
- FA 3 antibody
- FA3 antibody
see all
Images
-
Western blot - Anti-FANCC antibody (ab97575)Anti-FANCC antibody (ab97575) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Western blot - Anti-FANCC antibody (ab97575)Anti-FANCC antibody (ab97575) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab97575)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97575 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
References
This product has been referenced in:
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). Read more (PubMed: 23633493) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"