Anti-FANCC antibody (ab97575)

Submit a review Q&A (1)References (2)

Overview

  • Product name

    Anti-FANCC antibody
    See all FANCC primary antibodies

  • Description

    Rabbit polyclonal to FANCC

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 275 of FANCC (Q00597).

  • Positive control

    • WB: H1299, NIH 3T3, 293T, A431, HepG2 or Raji cell lysate; ICC/IF: HeLa cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

  • Developmental stage

    Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).

  • Cellular localization

    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt

  • Database links

    • Alternative names

      • bA80I15.1 antibody
      • FA 3 antibody
      • FA3 antibody
      • FAC antibody
      • FACC antibody
      • FANCC antibody
      • FANCC_HUMAN antibody
      • Fanconi anemia complementation group C antibody
      • Fanconi anemia complementation group C protein antibody
      • Fanconi anemia group C protein antibody
      • Fanconi pancytopenia type 3 antibody
      • FLJ14675 antibody
      • Protein FACC antibody
      see all

    Images

    • Western blot - Anti-FANCC antibody (ab97575)
      Western blot - Anti-FANCC antibody (ab97575)
      Anti-FANCC antibody (ab97575) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 63 kDa



      7.5% SDS Page
    • Western blot - Anti-FANCC antibody (ab97575)
      Western blot - Anti-FANCC antibody (ab97575)
      Anti-FANCC antibody (ab97575) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

      Predicted band size: 63 kDa



      7.5% SDS Page
    • Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab97575)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab97575)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97575 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.

    References

    This product has been referenced in:

    • Richardson CD  et al. CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. Nat Genet 50:1132-1139 (2018). Read more (PubMed: 30054595) »
    • Shen C  et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). Read more (PubMed: 23633493) »
    See all 2 Publications for this product

    Publishing research using ab97575? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I was having trouble with ab97575 in WB and was issued a replacement with ab5065. This also is not working to detect the human recombinant protein in WB. I would like a refund.

    Read More
    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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