Anti-FANCC antibody (ab97575)
Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FANCC antibody
See all FANCC primary antibodies -
Description
Rabbit polyclonal to FANCC -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 275 of FANCC (Q00597).
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Positive control
- WB: H1299, NIH 3T3, 293T, A431, HepG2 or Raji cell lysate; ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 63 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Developmental stage
Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level). -
Cellular localization
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 2176 Human
- Entrez Gene: 14088 Mouse
- Omim: 613899 Human
- SwissProt: Q00597 Human
- SwissProt: P50652 Mouse
- Unigene: 494529 Human
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Alternative names
- bA80I15.1 antibody
- FA 3 antibody
- FA3 antibody
see all
Images
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Western blot - Anti-FANCC antibody (ab97575)Anti-FANCC antibody (ab97575) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Western blot - Anti-FANCC antibody (ab97575)Anti-FANCC antibody (ab97575) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page -
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab97575)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97575 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
Protocols
References (2)
ab97575 has been referenced in 2 publications.
- Richardson CD et al. CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. Nat Genet 50:1132-1139 (2018). PubMed: 30054595
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). PubMed: 23633493
