Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FANCC antibody
See all FANCC primary antibodies
DescriptionRabbit polyclonal to FANCC
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant protein fragment corresponding to a region within amino acids 1 and 275 of FANCC (Q00597).
- WB: H1299, NIH 3T3, 293T, A431, HepG2 or Raji cell lysate; ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 63 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
Involvement in diseaseDefects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
Developmental stageExpression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
- Information by UniProt
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Anti-FANCC antibody (ab97575) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page
Anti-FANCC antibody (ab97575) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS Page
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97575 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
ab97575 has been referenced in 2 publications.
- Richardson CD et al. CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway. Nat Genet 50:1132-1139 (2018). PubMed: 30054595
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). PubMed: 23633493