Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab105023 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab105023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 59 kDa. Preliminary experiments gave an approx 70kDa band in Human Adipose and Uterus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.7kDa according to NP_068741.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.|
|IHC-P||Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAs part of the Fanconi anemia (FA) complex functions in DNA cross-links repair. Required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2.
Involvement in diseaseFanconi anemia complementation group E
modificationsPhosphorylated. Phosphorylation by CHEK1 at Thr-346 and Ser-374 regulates its function in DNA cross-links repair.
Ubiquitinated. Phosphorylation by CHEK1 induces polyubiquitination and degradation.
- Information by UniProt
- 2810451D06Rik antibody
- AI415634 antibody
- AW209126 antibody
ab105023 has not yet been referenced specifically in any publications.