Overview

  • Product name
  • Description
    Goat polyclonal to FANCF
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human FANCF aa 217-228 (Cysteine residue).
    Sequence:

    C-RPQEELEPGIHK


    Database link: Q9NPI8

  • Positive control
    • WB: HEK-293 cell lysate over expressing recombinant human FANCF.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • Involvement in disease
    Defects in FANCF are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A730016A17 antibody
    • FACF antibody
    • FAF antibody
    • FANCF antibody
    • FANCF_HUMAN antibody
    • Fanconi anemia complementation group F antibody
    • Fanconi anemia group F protein antibody
    • MGC126856 antibody
    • Protein FACF antibody
    • RGD1561456 antibody
    see all

Images

  • Lane 1 : Anti-IL-27-A antibody [EPR18247-101] (ab222648) at 1 µg/ml
    Lane 2 : Anti-FANCF antibody (ab223648) at 1 mg/ml
    Lane 3 : Anti-MYC Tag at 1/1000 dilution

    Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing Human FANCF with C-terminal MYC tag
    Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Primary incubations were for 1 hour

References

ab223648 has not yet been referenced specifically in any publications.

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