Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).|
FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Involvement in diseaseDefects in FANCF are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
- Information by UniProt
- A730016A17 antibody
- FACF antibody
- FAF antibody
Lane 1 : Anti-IL-27-A antibody [EPR18247-101] (ab222648) at 1 µg/ml
Lane 2 : Anti-FANCF antibody (ab223648) at 1 mg/ml
Lane 3 : Anti-MYC Tag at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing Human FANCF with C-terminal MYC tag
Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubations were for 1 hour
ab223648 has not yet been referenced specifically in any publications.