Anti-FANCG antibody (ab5067)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-FANCG antibody
See all FANCG primary antibodies -
DescriptionRabbit polyclonal to FANCG
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide:
MSRQTTSVGSSC
, corresponding to amino acids 1 - 12 of Human FANCG. -
Positive control
- Recombinant Human FANCG protein (ab114925) can be used as a positive control in WB.
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General notesAvoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab5067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
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Tissue specificityHighly expressed in testis and thymus. Found in lymphoblasts.
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Involvement in diseaseDefects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
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Sequence similaritiesContains 4 TPR repeats.
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Cellular localizationNucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
- Information by UniProt
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Database links
- Entrez Gene: 2189 Human
- Omim: 602956 Human
- SwissProt: O15287 Human
- Unigene: 591084 Human
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Alternative names
- DNA repair protein XRCC9 antibody
- FAG antibody
- FANCG antibody
see all
Images
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Western blot - Anti-FANCG antibody (ab5067)Anti-FANCG antibody (ab5067) at 1/500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 11 kDa, 24 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Song ZP et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin 37:753-62 (2016). Read more (PubMed: 27157092) »
