Anti-FANCG antibody (ab5067)

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Western blot - Anti-FANCG antibody (ab5067)

    Key features and details

    • Rabbit polyclonal to FANCG
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-FANCG antibody
      See all FANCG primary antibodies

    • Description

      Rabbit polyclonal to FANCG

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Synthetic peptide:

      MSRQTTSVGSSC

      , corresponding to amino acids 1 - 12 of Human FANCG.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Recombinant Human FANCG protein (ab114925) can be used as a positive control in WB.

    • General notes

      Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5067 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
    Notes
    WB
    1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Target

    • Function

      DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

    • Tissue specificity

      Highly expressed in testis and thymus. Found in lymphoblasts.

    • Involvement in disease

      Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.

    • Sequence similarities

      Contains 4 TPR repeats.

    • Cellular localization

      Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
    • Information by UniProt

    • Database links

      • Alternative names

        • DNA repair protein XRCC9 antibody
        • FAG antibody
        • FANCG antibody
        • FANCG_HUMAN antibody
        • Fanconi anaemia complementation group G antibody
        • Fanconi anemia group G protein antibody
        • Protein FACG antibody
        • X ray repair, complementing defective, in Chinese hamster cells 9 antibody
        • X-ray repair, complementing defective, in Chinese hamster, 9 antibody
        • XRCC9 antibody
        see all

      Images

      • Western blot - Anti-FANCG antibody (ab5067)
        Western blot - Anti-FANCG antibody (ab5067)
        Anti-FANCG antibody (ab5067) at 1/500 dilution + HeLa whole cell lysate at 35 µg

        Secondary
        IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 69 kDa
        Observed band size: 69 kDa
        Additional bands at: 11 kDa, 24 kDa. We are unsure as to the identity of these extra bands.

      References (2)

      Publishing research using ab5067? Please let us know so that we can cite the reference in this datasheet.

      ab5067 has been referenced in 2 publications.

      • Gonsalvez DG  et al. Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy. Front Mol Neurosci 12:275 (2019). PubMed: 31803018
      • Song ZP  et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin 37:753-62 (2016). PubMed: 27157092

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