Anti-FANCG antibody (ab5067)
Key features and details
- Rabbit polyclonal to FANCG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCG antibody
See all FANCG primary antibodies -
Description
Rabbit polyclonal to FANCG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
MSRQTTSVGSSC
, corresponding to amino acids 1 - 12 of Human FANCG. -
Positive control
- Recombinant Human FANCG protein (ab114925) can be used as a positive control in WB.
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General notes
Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| Notes |
|---|
|
WB
1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. -
Tissue specificity
Highly expressed in testis and thymus. Found in lymphoblasts. -
Involvement in disease
Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
Sequence similarities
Contains 4 TPR repeats. -
Cellular localization
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 2189 Human
- Omim: 602956 Human
- SwissProt: O15287 Human
- Unigene: 591084 Human
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Alternative names
- DNA repair protein XRCC9 antibody
- FAG antibody
- FANCG antibody
see all
Images
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Western blot - Anti-FANCG antibody (ab5067)Anti-FANCG antibody (ab5067) at 1/500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 11 kDa, 24 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (2)
ab5067 has been referenced in 2 publications.
- Gonsalvez DG et al. Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy. Front Mol Neurosci 12:275 (2019). PubMed: 31803018
- Song ZP et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin 37:753-62 (2016). PubMed: 27157092
