Overview

  • Product name
    Anti-FANCI antibody
    See all FANCI primary antibodies
  • Description
    Rabbit polyclonal to FANCI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human FANCI aa 200-250. The exact sequence is proprietary.
    Database link: Q9NVI1

  • Positive control
    • WB: HeLa cell nuclear extract. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19465922
IP Use at 1-4 µg/mg of lysate.
WB 1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).

Target

  • Function
    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • Involvement in disease
    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • Domain
    The C-terminal 30 residues are probably required for function in DNA repair.
  • Post-translational
    modifications
    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • Cellular localization
    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • Database links
    • Alternative names
      • FANCI antibody
      • FANCI gene antibody
      • FANCI_HUMAN antibody
      • Fanconi anemia group I protein antibody
      • Fanconi anemia, complementation group I antibody
      • FLJ10719 antibody
      • FLJ14658 antibody
      • KIAA1794 antibody
      • Protein FACI antibody
      • Protein FANCI antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody (ab15344)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody (ab15344)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
      ab15344 (1/400) staining FANCI in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

      See Abreview

    • Western blot - Anti-FANCI antibody (ab15344)
      Western blot - Anti-FANCI antibody (ab15344)
      All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution

      Lane 1 : HeLa nuclear extract at 5 µg
      Lane 2 : HeLa nuclear extract at 20 µg

      Developed using the ECL technique.

      Predicted band size: 149, 142, 27 kDa


      Exposure time: 15 minutes

    References

    This product has been referenced in:
    • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). Read more (PubMed: 28575672) »
    • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . Read more (PubMed: 23911284) »

    See all 7 Publications for this product

    Publishing research using ab15344? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    8 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-FANCI antibody

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 31 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

     Good
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2014

    ReviewID: xxx AbID: 15344, Anti-FANCI antibody Rating: Poor Image: Anti-FANCI antibody for Immunoprecipitation (Human) Sample: Species: Human Type: Cell lysate - whole cell Total protein in input:: 40 µg Specification: HeLa cell line Application:...

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    Thank you for submitting an Abreview of ab15344. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

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    Immunoprecipitation abreview for Anti-FANCI antibody

     Poor
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cell line)
    Total protein in input
    40 µg
    Specification
    HeLa cell line
    Immuno-precipitation step
    Protein G
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    I am a postdoc at the MGH. I saw on the website you have 3 antibodies for FANCI (ab15344, ab15346, ab74322). Would it be possible to have an aliquot to test how it works in my system for immunofluorescence and wb? We work mostly on mouse cells and sinc...

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    Thank you for your interest in our antibodies. We do not provide samples of our antibodies as under our Abpromise we guarantee our antibodies to work as stated on the data sheet. In the case of ab15344, ab74332 and ab15346as they are only guaranteed to...

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Read More
    abreview image
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 14 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 28 2011

    Western blot abreview for Anti-FANCI antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    40 µg
    Specification
    HeLa
    Treatment
    0.5µg/mL MMC for 24h
    Gel Running Conditions
    Reduced Denaturing (6%)
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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