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    fanci-antibody-ab15344.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-FANCI antibody (ab15344)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (8)

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Western blot - Anti-FANCI antibody (ab15344)

    Key features and details

    • Rabbit polyclonal to FANCI
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-FANCI antibody
      See all FANCI primary antibodies
    • Description

      Rabbit polyclonal to FANCI
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human FANCI aa 200-250. The exact sequence is proprietary.
      Database link: Q9NVI1

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Fanconi's Anemia

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human FANCI protein (ab132153)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab15344 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Chimpanzee
    Rhesus monkey
    Gorilla
    Orangutan
    Application Abreviews Notes
    WB (2)
    1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).
    Notes
    WB
    1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).

    Target

    • Function

      Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
    • Involvement in disease

      Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • Domain

      The C-terminal 30 residues are probably required for function in DNA repair.
    • Post-translational
      modifications

      Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
      Phosphorylated in response to DNA damage by ATM and/or ATR.
    • Cellular localization

      Nucleus. Concentrates in nuclear foci upon genotoxic stress.
    • Target information above from: UniProt accession Q9NVI1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55215 Human
      • Omim: 611360 Human
      • SwissProt: Q9NVI1 Human
      • Unigene: 513126 Human
      • Alternative names

        • FANCI antibody
        • FANCI gene antibody
        • FANCI_HUMAN antibody
        • Fanconi anemia group I protein antibody
        • Fanconi anemia, complementation group I antibody
        • FLJ10719 antibody
        • FLJ14658 antibody
        • KIAA1794 antibody
        • Protein FACI antibody
        • Protein FANCI antibody
        see all

      Images

      • Western blot - Anti-FANCI antibody (ab15344)
        Western blot - Anti-FANCI antibody (ab15344)
        All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution

        Lane 1 : HeLa nuclear extract at 5 µg
        Lane 2 : HeLa nuclear extract at 20 µg

        Developed using the ECL technique.

        Predicted band size: 149, 142, 27 kDa


        Exposure time: 15 minutes

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (8)

      Publishing research using ab15344? Please let us know so that we can cite the reference in this datasheet.

      ab15344 has been referenced in 8 publications.

      • Jin L  et al. MAST1 Drives Cisplatin Resistance in Human Cancers by Rewiring cRaf-Independent MEK Activation. Cancer Cell 34:315-330.e7 (2018). PubMed: 30033091
      • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). PubMed: 28575672
      • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . PubMed: 23911284
      • Nalepa G  et al. Fanconi anemia signaling network regulates the spindle assembly checkpoint. J Clin Invest 123:3839-47 (2013). WB ; Human . PubMed: 23934222
      • Mason JM & Sekiguchi JM Snm1B/Apollo functions in the Fanconi anemia pathway in response to DNA interstrand crosslinks. Hum Mol Genet 20:2549-59 (2011). WB ; Human . PubMed: 21478198
      • Lu WT  et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
      • Chan KL  et al. Replication stress induces sister-chromatid bridging at fragile site loci in mitosis. Nat Cell Biol 11:753-60 (2009). ICC/IF ; Human . PubMed: 19465922
      • Jamsai D  et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis 5:e979061 (0). PubMed: 26413409

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Western blot abreview for Anti-FANCI antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      10 µg
      Gel Running Conditions
      Reduced Denaturing (4-12 Bis tris)
      Sample
      Human Cell lysate - whole cell (293T)
      Specification
      293T
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 31 2014

      Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Permeabilization
      Yes - 0.5% Triton X100
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 25 2014

      Question

      ReviewID: xxx AbID: 15344, Anti-FANCI antibody Rating: Poor Image: Anti-FANCI antibody for Immunoprecipitation (Human) Sample: Species: Human Type: Cell lysate - whole cell Total protein in input:: 40 µg Specification: HeLa cell line Application: Application: Immuno-precipitation step: Protein G Other detail: Concentration: 4 µg/mg lysate Incubation time: 16 hours at 4°C Diluent: NTEN Buffer Western Blot Antibody: Name: Abcam antibody: Anti-FANCI antibody Conjugation: Dilution: 1/2000 Additional Data: Additional Notes: Lane 1: Ladder; Lane 2: input; Lane 3: IP using rabbit IgG; Lane 4: IP using ab15344.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 01 2012

      Answer

      Thank you for submitting an Abreview of ab15344. As you may have noticed, your review has now been published on our website.

      Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

      Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

      - Try titrating the antibody since this is an empirical factor that depends on the antibody as well as the sample.

      - As for the review, could you please indicate the MW markers? Right now we are not sure where you would be expecting the FANCI band (approx 145 kDa). Does the input show the expected band?

      If you decide to follow these suggestions, please let me know if they are helpful. Additionally, this antibody is covered by our Abpromise, so if the suggestions do not help and the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.
      Please let me know your order or PO number in that regard.

      I look forward to your reply.

      Read More

      Abcam Scientific Support

      Answered on Oct 01 2012

      Immunoprecipitation abreview for Anti-FANCI antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (HeLa cell line)
      Total protein in input
      40 µg
      Specification
      HeLa cell line
      Immuno-precipitation step
      Protein G
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 28 2012

      Question

      I am a postdoc at the MGH. I saw on the website you have 3 antibodies for FANCI (ab15344, ab15346, ab74322). Would it be possible to have an aliquot to test how it works in my system for immunofluorescence and wb? We work mostly on mouse cells and since looking at the sequences of the immunogenic peptide the antibodies are generated against they don’t look the most conserved regions it would be of great help to test it before ordering it.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 02 2011

      Answer

      Thank you for your interest in our antibodies. We do not provide samples of our antibodies as under our Abpromise we guarantee our antibodies to work as stated on the data sheet. In the case of ab15344, ab74332 and ab15346as they are only guaranteed to work in human samples and have not been tested on mouse, then I can offer you our testing discount program. Please see the link below for details on our testing program:   https://www.abcam.com/index.html\pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount If you would be interested in testing any of the above antibodies then please let me know Out of the three antibodies you selected, then ab15344 would be the best choice as it has been proven to work in WB and ICC/IF. If there is anything else I can help you with do not hesitate to ask.  

      Read More

      Abcam Scientific Support

      Answered on Dec 02 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Triton-X100 in PBS
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Nov 14 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-FANCI antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Fixative
      Formaldehyde
      Permeabilization
      Yes - 0.5% Triton X-100
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 28 2011

      Western blot abreview for Anti-FANCI antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa)
      Loading amount
      40 µg
      Specification
      HeLa
      Treatment
      0.5µg/mL MMC for 24h
      Gel Running Conditions
      Reduced Denaturing (6%)
      Blocking step
      BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 17 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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