Anti-FANCI antibody (ab15344)
Key features and details
- Rabbit polyclonal to FANCI
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCI antibody
See all FANCI primary antibodies -
Description
Rabbit polyclonal to FANCI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human FANCI aa 200-250. The exact sequence is proprietary.
Database link: Q9NVI1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa).
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| Notes |
|---|
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WB
1/2500 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 149, 142, 27 kDa). |
Target
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Function
Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites. -
Involvement in disease
Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Domain
The C-terminal 30 residues are probably required for function in DNA repair. -
Post-translational
modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR. -
Cellular localization
Nucleus. Concentrates in nuclear foci upon genotoxic stress. - Information by UniProt
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Database links
- Entrez Gene: 55215 Human
- Omim: 611360 Human
- SwissProt: Q9NVI1 Human
- Unigene: 513126 Human
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Alternative names
- FANCI antibody
- FANCI gene antibody
- FANCI_HUMAN antibody
see all
Images
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Western blot - Anti-FANCI antibody (ab15344)All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Developed using the ECL technique.
Predicted band size: 149, 142, 27 kDa
Exposure time: 15 minutes
Protocols
References (8)
ab15344 has been referenced in 8 publications.
- Jin L et al. MAST1 Drives Cisplatin Resistance in Human Cancers by Rewiring cRaf-Independent MEK Activation. Cancer Cell 34:315-330.e7 (2018). PubMed: 30033091
- Tan SLW et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). PubMed: 28575672
- Pourfarzad F et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . PubMed: 23911284
- Nalepa G et al. Fanconi anemia signaling network regulates the spindle assembly checkpoint. J Clin Invest 123:3839-47 (2013). WB ; Human . PubMed: 23934222
- Mason JM & Sekiguchi JM Snm1B/Apollo functions in the Fanconi anemia pathway in response to DNA interstrand crosslinks. Hum Mol Genet 20:2549-59 (2011). WB ; Human . PubMed: 21478198
- Lu WT et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
- Chan KL et al. Replication stress induces sister-chromatid bridging at fragile site loci in mitosis. Nat Cell Biol 11:753-60 (2009). ICC/IF ; Human . PubMed: 19465922
- Jamsai D et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis 5:e979061 (0). PubMed: 26413409
