Anti-FANCI antibody (ab74332)
Key features and details
- Rabbit polyclonal to FANCI
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCI antibody
See all FANCI primary antibodies -
Description
Rabbit polyclonal to FANCI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to a region between residue 1025 and 1075 of human KIAA1794 (NP_060663.2)
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Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 149 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites. -
Involvement in disease
Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Domain
The C-terminal 30 residues are probably required for function in DNA repair. -
Post-translational
modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR. -
Cellular localization
Nucleus. Concentrates in nuclear foci upon genotoxic stress. - Information by UniProt
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Database links
- Entrez Gene: 55215 Human
- Omim: 611360 Human
- SwissProt: Q9NVI1 Human
- Unigene: 513126 Human
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Alternative names
- FANCI antibody
- FANCI gene antibody
- FANCI_HUMAN antibody
see all
Images
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All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg
Lane 2 : Whole HeLa cell lysate at 15 µg
Lane 3 : Whole HeLa cell lysate at 5 µg
Lane 4 : Whole 293T cell lysate at 50 µg
Predicted band size: 149 kDa
Additional bands at: 100 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab74332 has been referenced in 5 publications.
- Zhang J et al. UBE2T regulates FANCI monoubiquitination to promote NSCLC progression by activating EMT. Oncol Rep 48:N/A (2022). PubMed: 35703356
- Jaber S et al. p53 downregulates the Fanconi anaemia DNA repair pathway. Nat Commun 7:11091 (2016). WB . PubMed: 27033104
- Luebben SW et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). ICC/IF ; Mouse . PubMed: 24589582
- Jamsai D et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . PubMed: 23451117
- Luebben SW et al. Helq acts in parallel to Fancc to suppress replication-associated genome instability. Nucleic Acids Res N/A:N/A (2013). PubMed: 24005041