Overview

  • Product name

    Anti-FANCI (phospho S556) antibody
    See all FANCI primary antibodies
  • Description

    Rabbit polyclonal to FANCI (phospho S556)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Goat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human FANCI (phospho S556).
    Database link: Q9NVI1

  • Positive control

    • WB: HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 149 kDa.

Target

  • Function

    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • Involvement in disease

    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • Domain

    The C-terminal 30 residues are probably required for function in DNA repair.
  • Post-translational
    modifications

    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • Cellular localization

    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • Database links

  • Alternative names

    • FANCI antibody
    • FANCI gene antibody
    • FANCI_HUMAN antibody
    • Fanconi anemia group I protein antibody
    • Fanconi anemia, complementation group I antibody
    • FLJ10719 antibody
    • FLJ14658 antibody
    • KIAA1794 antibody
    • Protein FACI antibody
    • Protein FANCI antibody
    see all

Images

  • All lanes : Anti-FANCI (phospho S556) antibody (ab228516) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with lambda phosphatase (800 units/1mg protein for 30 min)

    Predicted band size: 149 kDa



    Phosphospecificity is shown in Lane 2 where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1mg protein for 30 min).

References

ab228516 has not yet been referenced specifically in any publications.

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