Key features and details
- Rabbit polyclonal to FANCI (phospho S556)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FANCI (phospho S556) antibody
See all FANCI primary antibodies
DescriptionRabbit polyclonal to FANCI (phospho S556)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Goat, Cow
Synthetic peptide corresponding to Human FANCI (phospho S556).
Database link: Q9NVI1
- WB: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228516 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Our Abpromise guarantee covers the use of ab228516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 149 kDa.|
FunctionRequired for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
Involvement in diseaseDefects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
DomainThe C-terminal 30 residues are probably required for function in DNA repair.
modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR.
Cellular localizationNucleus. Concentrates in nuclear foci upon genotoxic stress.
- Information by UniProt
- FANCI antibody
- FANCI gene antibody
- FANCI_HUMAN antibody
All lanes : Anti-FANCI (phospho S556) antibody (ab228516) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with lambda phosphatase (800 units/1mg protein for 30 min)
Predicted band size: 149 kDa
Phosphospecificity is shown in Lane 2 where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1mg protein for 30 min).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228516 has not yet been referenced specifically in any publications.