Overview

  • Product name

  • Description

    Goat polyclonal to FANCL
  • Host species

    Goat
  • Specificity

    ab105020 is expected to recognize both isoforms (NP_001108108.1; NP_060532.2).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-QNLKDVLEIDFP

    , corresponding to internal sequence amino acids 282-293 of Human FANCL (NP_060532.2).

  • Positive control

    • Human Bone Marrow lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab105020 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Fanconi anemia complementation group L
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
  • Post-translational
    modifications

    The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase FANCL antibody
    • FAAP43 antibody
    • Fancl antibody
    • FANCL_HUMAN antibody
    • Fanconi anemia complementation group L antibody
    • Fanconi anemia group L protein antibody
    • Fanconi anemia-associated polypeptide of 43 kDa antibody
    • FLJ10335 antibody
    • PHD finger protein 9 antibody
    • PHF9 antibody
    • POG antibody
    • Ubiquitin ligase protein FANCL antibody
    see all

Images

  • Anti-FANCL antibody (ab105020) at 1 µg/ml + Human Bone Marrow lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    Primary incubation was 1 hour.

References

ab105020 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab105020.
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