Anti-FANCM antibody (ab167214)

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Overview

  • Product name

    Anti-FANCM antibody
    See all FANCM primary antibodies

  • Description

    Mouse polyclonal to FANCM

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Arabidopsis thaliana

  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-669 of Human FANCM (AAH36056.1, UniProt ID: Q8IYD8-2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human FANCM protein (ab132334) can be used as a positive control in WB. Lysate from 293T cells transfected with FANCM; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab167214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance

    FANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • ATP-dependent RNA helicase FANCM antibody
      • FAAP250 antibody
      • Fanconi anemia group M protein antibody
      • fanconi anemia-associated polypeptide of 250 kDa antibody
      • KIAA1596 antibody
      • Protein Hef ortholog antibody
      see all

    Images

    • Western blot - Anti-FANCM antibody (ab167214)
      Western blot - Anti-FANCM antibody (ab167214)
      All lanes : Anti-FANCM antibody (ab167214) at 1 µg/ml

      Lane 1 : Lysate from 293T cells transfected with FANCM
      Lane 2 : Non-transfected 293T cell lysate

      Predicted band size: 76 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-FANCM antibody (ab167214)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCM antibody (ab167214)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FANCM using ab167214 at 10 µg/ml.

    References

    ab167214 has not yet been referenced specifically in any publications.

    Publishing research using ab167214? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-FANCM antibody

     Poor
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Loading amount
    900 µg
    Gel Running Conditions
    Reduced Denaturing (6%, Laemmli)
    Sample
    Arabidopsis thaliana Tissue lysate - whole (closed flower buds)
    Specification
    closed flower buds
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Natasha Elina

    Verified customer

    Submitted Mar 04 2014

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