Anti-FANCM antibody (ab167214)
Key features and details
- Mouse polyclonal to FANCM
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FANCM antibody
See all FANCM primary antibodies -
Description
Mouse polyclonal to FANCM -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Arabidopsis thaliana -
Immunogen
Recombinant full length protein corresponding to amino acids 1-669 of Human FANCM (AAH36056.1, UniProt ID: Q8IYD8-2).
-
Positive control
- Recombinant Human FANCM protein (ab132334) can be used as a positive control in WB. Lysate from 293T cells transfected with FANCM; HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab167214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
-
Relevance
FANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 57697 Human
- Omim: 609644 Human
- SwissProt: Q8IYD8 Human
- Unigene: 509229 Human
-
Alternative names
- ATP-dependent RNA helicase FANCM antibody
- FAAP250 antibody
- Fanconi anemia group M protein antibody
see all
Images
-
Western blot - Anti-FANCM antibody (ab167214)All lanes : Anti-FANCM antibody (ab167214) at 1 µg/ml
Lane 1 : Lysate from 293T cells transfected with FANCM
Lane 2 : Non-transfected 293T cell lysate
Predicted band size: 76 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FANCM antibody (ab167214)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FANCM using ab167214 at 10 µg/ml.
Protocols
Datasheets and documents
References (0)
ab167214 has not yet been referenced specifically in any publications.
