Key features and details
- Rabbit polyclonal to FANCM
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FANCM antibody
See all FANCM primary antibodies
DescriptionRabbit polyclonal to FANCM
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
- HeLa whole cell lysate (ab150035)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95014 was affinity purified using an epitope specific to FANCM immobilized on solid support.
Our Abpromise guarantee covers the use of ab95014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 227 kDa.|
|IP||Use at 5-15 µg/mg of lysate.|
RelevanceFANCM is an ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. It binds to ssDNA but not to dsDNA.
- ATP-dependent RNA helicase FANCM antibody
- FAAP250 antibody
- Fanconi anemia group M protein antibody
All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 227 kDa
Exposure time: 10 seconds
ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP.
Lane 1: ab95014 at 10 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
ab95014 has been referenced in 3 publications.
- Chang EY et al. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts. Nat Commun 10:4265 (2019). PubMed: 31537797
- Chang EY et al. RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability. J Cell Biol 216:3991-4005 (2017). PubMed: 29042409
- Jamsai D et al. Uncoupling of transcription and translation of Fanconi anemia (FANC) complex proteins during spermatogenesis. Spermatogenesis 5:e979061 (0). PubMed: 26413409