Recombinant Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP325] to Fibroblast activation protein, alpha
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibroblast activation protein, alpha antibody [SP325]
See all Fibroblast activation protein, alpha primary antibodies -
Description
Rabbit monoclonal [SP325] to Fibroblast activation protein, alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human melanoma, colon adenocarcinoma, ovarian adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma, skin squamous cell carcinoma and cervical squamous cell carcinoma tissues. WB: HFF-1 cell lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP325 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (4) |
1/100.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 10 minutes at room temperature. |
WB |
1/0.176. Predicted molecular weight: 88 kDa.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 10 minutes at room temperature. |
WB
1/0.176. Predicted molecular weight: 88 kDa. |
Target
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Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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Database links
- Entrez Gene: 508882 Cow
- Entrez Gene: 2191 Human
- Omim: 600403 Human
- SwissProt: A5D7B7 Cow
- SwissProt: Q12884 Human
- Unigene: 654370 Human
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Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- 170 kDa melanoma membrane-bound gelatinase antibody
- DPPIV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab227703 at 1/100 dilution (1.76 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stroma in the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227703 for 10 mins at room temperature. -
Lane 1 : Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703) at 0.176 µg/ml (purified)
Lane 2 : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
Lane 1 : HFF-1 (Human Skin fibroblast) cell lysates
Lane 2 : HFF-1 (Human Skin fibroblast)
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg
Predicted band size: 88 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM /TBST
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human melanoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab227703 has been referenced in 4 publications.
- Zboralski D et al. Preclinical evaluation of FAP-2286 for fibroblast activation protein targeted radionuclide imaging and therapy. Eur J Nucl Med Mol Imaging 49:3651-3667 (2022). PubMed: 35608703
- Huang WC et al. The MEK/ERK/miR-21 Signaling Is Critical in Osimertinib Resistance in EGFR-Mutant Non-Small Cell Lung Cancer Cells. Cancers (Basel) 13:N/A (2021). PubMed: 34885115
- Hintz HM et al. Imaging Fibroblast Activation Protein Alpha Improves Diagnosis of Metastatic Prostate Cancer with Positron Emission Tomography. Clin Cancer Res 26:4882-4891 (2020). PubMed: 32636317
- Liu J et al. Stromal fibroblast activation protein alpha promotes gastric cancer progression via epithelial-mesenchymal transition through Wnt/ ß-catenin pathway. BMC Cancer 18:1099 (2018). PubMed: 30419872