Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    May function as Rho-guanine nucleotide exchange factor.
  • Tissue specificity
    Detected in cAMP-treated chondrocytes, but not in untreated chondrocytes. Detected in fetal brain, heart and spleen, and in adult testis, kidney and lung.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 FERM domain.
    Contains 2 PH domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDEP antibody
    • Chondrocyte derived ezrin like protein antibody
    • Chondrocyte-derived ezrin-like protein antibody
    • FARP1 antibody
    • FARP1_HUMAN antibody
    • FERM RhoGEF and pleckstrin domain containing protein 1 antibody
    • FERM antibody
    • FERM RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) antibody
    • FERM, ARHGEF, and pleckstrin domain protein 1 antibody
    • PH domain-containing family C member 2 antibody
    • Pleckstrin homology domain-containing family C member 2 antibody
    • PLEKHC2 antibody
    • PPP1R75 antibody
    • protein phosphatase 1, regulatory subunit 75 antibody
    • RhoGEF and pleckstrin domain-containing protein 1 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab27494 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

  • Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab27494 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab27494 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

References

ab27494 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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