Anti-FARSLA antibody (ab224049)
Key features and details
- Rabbit polyclonal to FARSLA
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-FARSLA antibody -
Description
Rabbit polyclonal to FARSLA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human FARSLA aa 1-200.
Database link: Q9Y285 -
Positive control
- IHC: Human duodenum tissue. WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysate; human liver and tonsil tissue lysate. ICC/IF: A431 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 58 kDa.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
---|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 58 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
-
Relevance
FARSLA (Phenylalanyl-tRNA synthetase alpha chain) is an aminoacyl-tRNA synthetase. These are a class of enzymes that charge tRNAs with their cognate amino acids. Cytoplasmic phenylalanine-tRNA synthetase is a heterodimer consisting of a catalytic alpha subunit, FARSLA, and a regulatory beta subunit, FARSLB. This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase family shown to exhibit this type of regulated expression. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 2193 Human
- Entrez Gene: 66590 Mouse
- Entrez Gene: 288917 Rat
- Omim: 602918 Human
- SwissProt: Q9Y285 Human
- SwissProt: Q8C0C7 Mouse
- SwissProt: Q5RFA2 Orangutan
- SwissProt: Q505J8 Rat
-
Alternative names
- CML 33 antibody
- CML33 antibody
- FARS antibody
see all
Images
-
Paraffin-embedded human duodenum tissue stained for FARSLA using ab224049 at 1/1000 dilution in immunohistochemical analysis.
-
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for FARSLA (green) using ab224049 at 4 µg/ml in ICC/IF.
-
All lanes : Anti-FARSLA antibody (ab224049) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 58 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab224049 has not yet been referenced specifically in any publications.