Overview

  • Product name

  • Description

    Rabbit polyclonal to FARSLB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal amino acids 378-560 of Human FARSLB (Q9NSD9).

  • Positive control

    • Human Cal27 xenograft tissue, Rat RT2 xenograft tissue; PC3, U-87 MG and SK N SH whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer ( PH8.0).

Target

  • Sequence similarities

    Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 2 subfamily.
    Contains 1 B5 domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C76708 antibody
    • Farsa antibody
    • FARSB antibody
    • Farsl antibody
    • Farslb antibody
    • FRSB antibody
    • HSPC173 antibody
    • PheHB antibody
    • Phenylalanine tRNA ligase beta subunit antibody
    • phenylalanine tRNA ligase 1, beta, cytoplasmic antibody
    • Phenylalanine tRNA ligase beta chain antibody
    • Phenylalanine--tRNA ligase beta chain antibody
    • phenylalanine-tRNA synthetase-like, beta subunit antibody
    • Phenylalanyl tRNA synthetase beta chain antibody
    • Phenylalanyl-tRNA synthetase beta chain antibody
    • phenylalanyl-tRNA synthetase beta-subunit antibody
    • phenylalanyl-tRNA synthetase-like, beta subunit antibody
    • PheRS antibody
    • SYFB_HUMAN antibody
    see all

Images

  • All lanes : Anti-FARSLB antibody (ab126950) at 1/1000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : U-87 MG cell lysate
    Lane 3 : SK N SH cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 66 kDa



    7.5% SDS Page
  • ab126950, at a 1/500 dilution, staining FARSLB in paraffin-embedded Human Cal27 xenograft tissue by immunohistochemistry.
  • ab126950, at a 1/500 dilution, staining FARSLB in paraffin-embedded Rat RT2 xenograft tissue by immunohistochemistry.

References

ab126950 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Murine cells)
Specification
Murine cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 10 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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