Anti-Fas antibody (ab82419)
Key features and details
- Rabbit polyclonal to Fas
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Fas antibody
See all Fas primary antibodies -
Description
Rabbit polyclonal to Fas -
Host species
Rabbit -
Specificity
Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Fas conjugated to keyhole limpet haemocyanin.
Database link: P25446 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
Tissue specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Domain
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 14102 Mouse
- Entrez Gene: 246097 Rat
- SwissProt: P25446 Mouse
- SwissProt: Q63199 Rat
- Unigene: 1626 Mouse
- Unigene: 162521 Rat
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Alternative names
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
Images
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Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous AbreviewAll lanes : Anti-Fas antibody (ab82419) at 1/3000 dilution
Lane 1 : Mouse brain whole tissue lysate
Lane 2 : Mouse retina whole tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running conditions:
Reduced Denaturing (4-12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s).
Concentration: 5% - Temperature: 22°C
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Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous Abreview.All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running Conditions:
Reduced Denaturing (4 -12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s). Concentration: 5% - Temperature: 4°C
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Western blot - Anti-Fas antibody (ab82419)All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : Rat brain tissue extract
Lane 2 : Mouse brain tissue extract
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (84)
ab82419 has been referenced in 84 publications.
- Liang X & Ju J Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK. Oncol Rep 45:N/A (2021). PubMed: 33786627
- He C et al. Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy. Yonsei Med J 62:215-223 (2021). PubMed: 33635011
- Fan L & Zhou L AG490 protects cerebral ischemia/reperfusion injury via inhibiting the JAK2/3 signaling pathway. Brain Behav 11:e01911 (2021). PubMed: 33098244
- Li C et al. Mutation of the novel acetylation site at K414R of BECN1 is involved in adipocyte differentiation and lipolysis. J Cell Mol Med 25:6855-6863 (2021). PubMed: 34085745
- Chen FX et al. Intramuscular accumulation of pentadecanoic acid activates AKT1 to phosphorylate NCOR1 and triggers FOXM1-mediated apoptosis in the pathogenesis of sarcopenia. Am J Transl Res 12:5064-5079 (2020). PubMed: 33042406