Anti-Fas antibody (ab82419)
Key features and details
- Rabbit polyclonal to Fas
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Fas antibody
See all Fas primary antibodies -
Description
Rabbit polyclonal to Fas -
Host species
Rabbit -
Specificity
Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab82419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
-
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
Tissue specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
Involvement in disease
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Domain
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 355 Human
- Entrez Gene: 14102 Mouse
- Entrez Gene: 246097 Rat
- Omim: 134637 Human
- SwissProt: P25445 Human
- SwissProt: P25446 Mouse
- SwissProt: Q63199 Rat
- Unigene: 244139 Human
see all -
Alternative names
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
Images
-
Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous AbreviewAll lanes : Anti-Fas antibody (ab82419) at 1/3000 dilution
Lane 1 : Mouse brain whole tissue lysate
Lane 2 : Mouse retina whole tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running conditions:
Reduced Denaturing (4-12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s).
Concentration: 5% - Temperature: 22°C
-
Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous Abreview.All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 5 minutesGel Running Conditions:
Reduced Denaturing (4 -12% BisTris Gel)
Blocking step:
Milk as blocking agent for 1 hour(s) and 0 minute(s). Concentration: 5% - Temperature: 4°C
-
Western blot - Anti-Fas antibody (ab82419)All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : Rat brain tissue extract
Lane 2 : Mouse brain tissue extract
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Protocols
Datasheets and documents
References (65)
ab82419 has been referenced in 65 publications.
- Huang Y et al. Resveratrol protects against nonalcoholic fatty liver disease by improving lipid metabolism and redox homeostasis via the PPARa pathway. Appl Physiol Nutr Metab 45:227-239 (2020). PubMed: 31173696
- Xu Q et al. Association of iRhom1 and iRhom2 expression with prognosis in patients with cervical cancer and possible signaling pathways. Oncol Rep 43:41-54 (2020). PubMed: 31661139
- Zhao Q et al. Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways. Int J Mol Sci 21:N/A (2020). PubMed: 32156008
- Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011
- Cremonese C et al. Short-Term Western Diet Aggravates Non-Alcoholic Fatty Liver Disease (NAFLD) With Portal Hypertension in TGR(mREN2)27 Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32392802
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com