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    fas-antibody-ab82419.pdf

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Cell Biology Apoptosis Receptors Death Receptors
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Anti-Fas antibody (ab82419)

  • Datasheet
Reviews (13)Q&A (1)References (65)

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Western blot - Anti-Fas antibody (ab82419)
  • Western blot - Anti-Fas antibody (ab82419)
  • Western blot - Anti-Fas antibody (ab82419)

Key features and details

  • Rabbit polyclonal to Fas
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Fas antibody [EPR5700] (ab133619)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Fas antibody
    See all Fas primary antibodies
  • Description

    Rabbit polyclonal to Fas
  • Host species

    Rabbit
  • Specificity

    Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily

Associated products

  • Compatible Secondaries

    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Rat brain cerebellum tissue lysate - total protein (ab4032)
  • Recombinant Protein

    • Recombinant human Fas protein (ab155635)
  • Related Products

    • Normal Donkey Serum (ab7475)

Applications

Our Abpromise guarantee covers the use of ab82419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificity

    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in disease

    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities

    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Domain

    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P25445 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 355 Human
    • Entrez Gene: 14102 Mouse
    • Entrez Gene: 246097 Rat
    • Omim: 134637 Human
    • SwissProt: P25445 Human
    • SwissProt: P25446 Mouse
    • SwissProt: Q63199 Rat
    • Unigene: 244139 Human
    • Unigene: 1626 Mouse
    • Unigene: 162521 Rat
    see all
  • Alternative names

    • ALPS 1A antibody
    • ALPS1A antibody
    • APO 1 antibody
    • Apo 1 antigen antibody
    • APO 1 cell surface antigen antibody
    • Apo-1 antigen antibody
    • APO1 antibody
    • Apo1 antigen antibody
    • APO1 cell surface antigen antibody
    • Apoptosis antigen 1 antibody
    • Apoptosis mediating surface antigen FAS antibody
    • Apoptosis-mediating surface antigen FAS antibody
    • APT 1 antibody
    • APT1 antibody
    • CD 95 antibody
    • CD 95 antigen antibody
    • CD95 antibody
    • CD95 antigen antibody
    • Delta Fas antibody
    • Delta Fas/APO 1/CD95 antibody
    • Delta Fas/APO1/CD95 antibody
    • Fas (TNF receptor superfamily, member 6) antibody
    • FAS 1 antibody
    • FAS 827dupA antibody
    • Fas AMA antibody
    • Fas antibody
    • FAS Antigen antibody
    • Fas cell surface death receptor antibody
    • FAS1 antibody
    • FASLG receptor antibody
    • FASTM antibody
    • sFAS antibody
    • Surface antigen APO1 antibody
    • TNF receptor superfamily, member 6 antibody
    • TNFRSF 6 antibody
    • TNFRSF6 antibody
    • TNR6_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody
    see all

Images

  • Western blot - Anti-Fas antibody (ab82419)
    Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous Abreview
    All lanes : Anti-Fas antibody (ab82419) at 1/3000 dilution

    Lane 1 : Mouse brain whole tissue lysate
    Lane 2 : Mouse retina whole tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 5 minutes


    Gel Running conditions:

    Reduced Denaturing (4-12% BisTris Gel)

    Blocking step:

    Milk as blocking agent for 1 hour(s) and 0 minute(s).

    Concentration: 5% - Temperature: 22°C

     

  • Western blot - Anti-Fas antibody (ab82419)
    Western blot - Anti-Fas antibody (ab82419)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : ECL Rabbit IgG, HRP-linked whole Ab from donkey at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 5 minutes


    Gel Running Conditions: 

    Reduced Denaturing (4 -12% BisTris Gel)

    Blocking step:

    Milk as blocking agent for 1 hour(s) and 0 minute(s). Concentration: 5% - Temperature: 4°C

  • Western blot - Anti-Fas antibody (ab82419)
    Western blot - Anti-Fas antibody (ab82419)
    All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution

    Lane 1 : Rat brain tissue extract
    Lane 2 : Mouse brain tissue extract

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (65)

    Publishing research using ab82419? Please let us know so that we can cite the reference in this datasheet.

    ab82419 has been referenced in 65 publications.

    • Huang Y  et al. Resveratrol protects against nonalcoholic fatty liver disease by improving lipid metabolism and redox homeostasis via the PPARa pathway. Appl Physiol Nutr Metab 45:227-239 (2020). PubMed: 31173696
    • Xu Q  et al. Association of iRhom1 and iRhom2 expression with prognosis in patients with cervical cancer and possible signaling pathways. Oncol Rep 43:41-54 (2020). PubMed: 31661139
    • Zhao Q  et al. Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways. Int J Mol Sci 21:N/A (2020). PubMed: 32156008
    • Wu Q  et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011
    • Cremonese C  et al. Short-Term Western Diet Aggravates Non-Alcoholic Fatty Liver Disease (NAFLD) With Portal Hypertension in TGR(mREN2)27 Rats. Int J Mol Sci 21:N/A (2020). PubMed: 32392802
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-10 of 13 Abreviews

    Western blot abreview for Anti-Fas antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela, HepG2)
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris Gel)
    Loading amount
    10 µg
    Specification
    Hela, HepG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 18 2019

    Western blot abreview for Anti-Fas antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain, retina)
    Gel Running Conditions
    Reduced Denaturing (4-12% BisTris Gel)
    Loading amount
    30 µg
    Specification
    brain, retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fas antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (Brain sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
    Permeabilization
    No
    Specification
    Brain sections
    Blocking step
    BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More

    Dr. Marcelo Pelajo Machado

    Verified customer

    Submitted Jul 21 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Tissue sections (Retina)
    Specification
    Retina
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD95 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 12 2013

    Immunocytochemistry abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (RAW267.4 cell line)
    Specification
    RAW267.4 cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    Immunocytochemistry abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Kelly Neuroblastoma cell line)
    Specification
    Kelly Neuroblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    Immunocytochemistry abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cell (33B Cell line)
    Specification
    33B Cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD95 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (B50 Neuroblastoma cell line)
    Specification
    B50 Neuroblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    1-10 of 13 Abreviews

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