- Datasheet
- Specific References (26)
- Protocols
Overview
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Product nameAnti-Fas antibody
See all Fas primary antibodies -
DescriptionRabbit polyclonal to Fas
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Host speciesRabbit
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SpecificityRecognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ICC, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Alligator
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Immunogen
Synthetic peptide (Mouse) derived from the extracellular domain, conjugated to KLH
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Positive control
- Rat and Mouse brain tissue extracs; Human hepatoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab82419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. PubMed: 16339059 | |
| ICC/IF | 1/50. | |
| IHC-P | 1/50. | |
| IHC-Fr | 1/50. | |
| ICC | 1/50. | |
| WB | 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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FunctionReceptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
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Tissue specificityIsoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
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Involvement in diseaseDefects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
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Sequence similaritiesContains 1 death domain.
Contains 3 TNFR-Cys repeats. -
DomainContains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
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Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 355 Human
- Entrez Gene: 14102 Mouse
- Entrez Gene: 246097 Rat
- Omim: 134637 Human
- SwissProt: P25445 Human
- SwissProt: P25446 Mouse
- SwissProt: Q63199 Rat
- Unigene: 244139 Human
see all -
Alternative names
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
Images
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Western blot - Anti-Fas antibody (ab82419)All lanes : Anti-Fas antibody (ab82419) at 1/1000 dilution
Lane 1 : Rat brain tissue extract
Lane 2 : Mouse brain tissue extract
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Immunohistochemistry (Frozen sections) - Anti-Fas antibody (ab82419)Image courtesy of an anonymous Abreview.ab82419 staining CD95 in rat spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in 4% paraformaldehyde, blocked with 10% donkey serum (ab7475) for 20 minutes at 24°C, then incubated with ab82419 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal (ab150073), used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody (ab82419)This image is courtesy of an anonymous Abreviewab82419 staining CD95 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) ab150073) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Song HM et al. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS. Int J Mol Med 42:193-207 (2018). Read more (PubMed: 29620148) »
- Wei X et al. GMDS knockdown impairs cell proliferation and survival in human lung adenocarcinoma. BMC Cancer 18:600 (2018). Read more (PubMed: 29843634) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"