Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

Overview

  • Product name

    Anti-Fas antibody [EPR5700] - BSA and Azide free
    See all Fas primary antibodies
  • Description

    Rabbit monoclonal [EPR5700] to Fas - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human CD95 (UniProt ID: P25445).

  • Positive control

    • Ramos, HT-1080, and Raji cell lysates, Human tonsil tissue
  • General notes

    Ab178076 is the carrier-free version of ab133619. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab178076 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab178076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
    • Tissue specificity

      Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
    • Involvement in disease

      Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.
    • Domain

      Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ALPS 1A antibody
      • ALPS1A antibody
      • APO 1 antibody
      • Apo 1 antigen antibody
      • APO 1 cell surface antigen antibody
      • Apo-1 antigen antibody
      • APO1 antibody
      • Apo1 antigen antibody
      • APO1 cell surface antigen antibody
      • Apoptosis antigen 1 antibody
      • Apoptosis mediating surface antigen FAS antibody
      • Apoptosis-mediating surface antigen FAS antibody
      • APT 1 antibody
      • APT1 antibody
      • CD 95 antibody
      • CD 95 antigen antibody
      • CD95 antibody
      • CD95 antigen antibody
      • Delta Fas antibody
      • Delta Fas/APO 1/CD95 antibody
      • Delta Fas/APO1/CD95 antibody
      • Fas (TNF receptor superfamily, member 6) antibody
      • FAS 1 antibody
      • FAS 827dupA antibody
      • Fas AMA antibody
      • Fas antibody
      • FAS Antigen antibody
      • Fas cell surface death receptor antibody
      • FAS1 antibody
      • FASLG receptor antibody
      • FASTM antibody
      • sFAS antibody
      • Surface antigen APO1 antibody
      • TNF receptor superfamily, member 6 antibody
      • TNFRSF 6 antibody
      • TNFRSF6 antibody
      • TNR6_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 6 antibody
      see all

    Images

    • Anti-Fas antibody [EPR5700] (ab133619)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Fas with ab133619 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
      This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD95 with ab133619 antibody at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133619).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab178076 has not yet been referenced specifically in any publications.

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