Overview

  • Product name

    Anti-FAS Intracellular Fragment antibody [LT95] (PE/Cy7 ®)
  • Description

    Mouse monoclonal [LT95] to FAS Intracellular Fragment (PE/Cy7 ®)
  • Host species

    Mouse
  • Conjugation

    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to FAS Intracellular Fragment. (HUT-78 human T cell lymphoma cell line)

  • Positive control

    • Flow Cytometry: Human peripheral blood.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    ab234275 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • Clonality

    Monoclonal
  • Clone number

    LT95
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

Target

  • Relevance

    Fas is a receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Isoform 1 is located at the cell membrane as a single-pass type I meembrane protein.
  • Cellular localization

    Cell Membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • Apoptosis mediating surface antigen FAS antibody
    • APT1 antibody
    • TNF receptor superfamily member 6 antibody
    • Tumor necrosis factor receptor superfamily member 6 antibody

Images

  • Flow Cytometry analysis of human peripheral blood labeling FAS Intracellular Fragment with ab234275. Surface staining. Gated on leukocytes.

References

ab234275 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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