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  1. Link

    fas-ligand-antibody-ab15285.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Anti-Fas Ligand antibody (ab15285)

  • Datasheet
  • SDS
Reviews (22)Q&A (7)References (72)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (ab15285)

    Key features and details

    • Rabbit polyclonal to Fas Ligand
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Fas Ligand antibody
      See all Fas Ligand primary antibodies
    • Description

      Rabbit polyclonal to Fas Ligand
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Synthetic peptide within Human Fas Ligand aa 1-100. The exact sequence is proprietary.
      Database link: P48023

    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Immunology
      • Innate Immunity
      • Cytokines
      • Necrosis Factors
      • Immunology
      • Innate Immunity
      • Cytokines
      • TNF Superfamily
      • Immunology
      • Immune System Diseases
      • Autoimmune
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Fas Ligand protein (ab157085)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab15285 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (1)
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
    • Involvement in disease

      Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Post-translational
      modifications

      N-glycosylated.
      The soluble form derives from the membrane form by proteolytic processing.
    • Cellular localization

      Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
    • Target information above from: UniProt accession P48023 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 356 Human
      • Entrez Gene: 14103 Mouse
      • Entrez Gene: 25385 Rat
      • Omim: 134638 Human
      • SwissProt: P48023 Human
      • SwissProt: P41047 Mouse
      • SwissProt: P36940 Rat
      • Unigene: 2007 Human
      • Unigene: 3355 Mouse
      • Unigene: 9725 Rat
      see all
    • Alternative names

      • ALPS1B antibody
      • Apoptosis (APO 1) antigen ligand 1 antibody
      • Apoptosis antigen ligand 1 antibody
      • Apoptosis antigen ligand antibody
      • APT1LG1 antibody
      • APTL antibody
      • CD178 antibody
      • CD178 antigen antibody
      • CD95 ligand antibody
      • CD95-L antibody
      • CD95L antibody
      • CD95L protein antibody
      • Fas antigen ligand antibody
      • Fas L antibody
      • Fas ligand (TNF superfamily member 6) antibody
      • Fas ligand antibody
      • FASL antibody
      • Fasl Fas ligand (TNF superfamily member 6) antibody
      • FASLG antibody
      • Generalized lymphoproliferative disease antibody
      • Gld antibody
      • soluble form antibody
      • TNFL6_HUMAN antibody
      • TNFSF6 antibody
      • Tumor necrosis factor (ligand) superfamily member 6 antibody
      • Tumor necrosis factor ligand superfamily member 6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (ab15285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (ab15285)

      ab15285 at 1/100 dilution staining Fas Ligand in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (72)

    Publishing research using ab15285? Please let us know so that we can cite the reference in this datasheet.

    ab15285 has been referenced in 72 publications.

    • Chen Y  et al. LncRNA LINC00324 is upregulated in intervertebral disk degeneration and upregulates FasL in nucleus pulposus cells. Mol Cell Biochem 476:1995-2000 (2021). PubMed: 33511550
    • Jin F  et al. A functional motif of long noncoding RNA Nron against osteoporosis. Nat Commun 12:3319 (2021). PubMed: 34083547
    • Liu C  et al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models. Cell Discov 6:15 (2020). PubMed: 32218989
    • Liu Y  et al. Active vitamin D supplementation alleviates initiation and progression of nonalcoholic fatty liver disease by repressing the p53 pathway. Life Sci 241:117086 (2020). PubMed: 31756344
    • Zhu Y  et al. Angiotensin II triggers RIPK3-MLKL-mediated necroptosis by activating the Fas/FasL signaling pathway in renal tubular cells. PLoS One 15:e0228385 (2020). PubMed: 32134954
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 29 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fas Ligand antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (Bone marrow)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6,0
    Permeabilization
    No
    Specification
    Bone marrow
    Blocking step
    BSA+MILK+BFS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 18 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Western blot abreview for Anti-Fas Ligand antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Mouse Tissue lysate - whole (uterus)
    Specification
    uterus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Varkha Agrawal

    Verified customer

    Submitted Nov 14 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Fas Ligand antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Placenta)
    Specification
    Placenta
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 13 2013

    Western blot abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat insulinoma cells)
    Loading amount
    20 µg
    Specification
    rat insulinoma cells
    Gel Running Conditions
    Reduced Denaturing (SDS boiling for 5 min)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human Retina)
    Specification
    Human Retina
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2012

    Immunocytochemistry abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (Kelly Neuroblastoma cell line)
    Specification
    Kelly Neuroblastoma cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 18 2012

    Western blot abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Neuro2a)
    Loading amount
    6 µg
    Specification
    Neuro2a
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 21 2012

    Western blot abreview for Anti-Fas Ligand antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (B50 cell line)
    Loading amount
    6 µg
    Specification
    B50 cell line
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 09 2012

    Immunocytochemistry abreview for Anti-Fas Ligand antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cultured Cells (33B)
    Specification
    33B
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    1-10 of 29 Abreviews or Q&A

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