- Datasheet
- Specific References (37)
- Protocols
Overview
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Product nameAnti-Fas Ligand antibody
See all Fas Ligand primary antibodies -
DescriptionRabbit polyclonal to Fas Ligand
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, ICC, ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Fas Ligand aa 1-100. The exact sequence is proprietary.
Database link: P48023 -
Positive control
- Prostate tissue slides.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab15285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/100. | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionCytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
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Involvement in diseaseDefects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsN-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
Cellular localizationCell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
- Information by UniProt
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Database links
- Entrez Gene: 356 Human
- Entrez Gene: 14103 Mouse
- Entrez Gene: 25385 Rat
- Omim: 134638 Human
- SwissProt: P48023 Human
- SwissProt: P41047 Mouse
- SwissProt: P36940 Rat
- Unigene: 2007 Human
see all -
Alternative names
- ALPS1B antibody
- Apoptosis (APO 1) antigen ligand 1 antibody
- Apoptosis antigen ligand 1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous Abreviewab15285 staining Fas Ligand in mouse skeletal muscle blood vessel tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/100 in PBS-Tween 20) at 4°C for 12 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/250) was used as secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas Ligand antibody (ab15285)ab15285 at 1/100 dilution staining Fas Ligand in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous Abreviewab15285 staining Fas Ligand in Mouse macrophage RAAW264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)This image is courtesy of an anonymous abreview.ab15285 staining Fas Ligand in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue). -
Immunocytochemistry/ Immunofluorescence - Anti-Fas Ligand antibody (ab15285)Image courtesy of an anonymous Abreviewab15285 staining Fas Ligand in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 12 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).
Protocols
References
This product has been referenced in:
- Gugliandolo A et al. Eruca sativa seed extract: A novel natural product able to counteract neuroinflammation. Mol Med Rep 17:6235-6244 (2018). Read more (PubMed: 29512782) »
- Qin L et al. MicroRNA-497 promotes proliferation and inhibits apoptosis of cardiomyocytes through the downregulation of Mfn2 in a mouse model of myocardial ischemia-reperfusion injury. Biomed Pharmacother 105:103-114 (2018). WB . Read more (PubMed: 29852387) »
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