Overview

  • Product name

  • Description

    Goat polyclonal to Fas Ligand
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Fas Ligand. CHO cell derived.
    Database link: P48023

  • Positive control

    • Human angiosarcoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab186671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.
Neutralising Use a concentration of 0.08 - 0.1 µg/ml.

ab186671 at a concentration of 0.08 - 0.1 µg/ml yields one-half maximal inhibition [ND50] of the biological activity of Fas Ligand (10 ng/ml).

IHC-P Use a concentration of 0.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

Target

  • Function

    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • Involvement in disease

    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization

    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALPS1B antibody
    • Apoptosis (APO 1) antigen ligand 1 antibody
    • Apoptosis antigen ligand 1 antibody
    • Apoptosis antigen ligand antibody
    • APT1LG1 antibody
    • APTL antibody
    • CD178 antibody
    • CD178 antigen antibody
    • CD95 ligand antibody
    • CD95-L antibody
    • CD95L antibody
    • CD95L protein antibody
    • Fas antigen ligand antibody
    • Fas L antibody
    • Fas ligand (TNF superfamily member 6) antibody
    • Fas ligand antibody
    • FASL antibody
    • Fasl Fas ligand (TNF superfamily member 6) antibody
    • FASLG antibody
    • Generalized lymphoproliferative disease antibody
    • Gld antibody
    • soluble form antibody
    • TNFL6_HUMAN antibody
    • TNFSF6 antibody
    • Tumor necrosis factor (ligand) superfamily member 6 antibody
    • Tumor necrosis factor ligand superfamily member 6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human angiosarcoma tissue, labeling Fas Ligand with ab186671 at 2.5 µg/ml. An HRP-labeled polymer detection system was used with a AB chromogen.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human angiosarcoma tissue, labeling Fas Ligand with ab186671 at 2.5 µg/ml. An HRP-labeled polymer detection system was used with a AB chromogen.

References

This product has been referenced in:

  • Zhou Z  et al. Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia-reperfusion injury via upregulation of the PI3K/Akt pathway. Mol Med Rep 18:1560-1570 (2018). Read more (PubMed: 29901074) »
See 1 Publication for this product

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