Anti-Fas Ligand antibody (ab186671)
Key features and details
- Goat polyclonal to Fas Ligand
- Suitable for: WB, IHC-P, Sandwich ELISA, Neutralising
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fas Ligand antibody
See all Fas Ligand primary antibodies -
Description
Goat polyclonal to Fas Ligand -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-P, Sandwich ELISA, Neutralisingmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Fas Ligand. CHO cell derived.
Database link: P48023 -
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab186671 was purified by affinity chromatography employing immobilized Human Fas Ligand/Apo1L matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
ab186671 at a concentration of 0.08 - 0.1 ug/ml yields one-half maximal inhibition [ND50] of the biological activity of Fas Ligand (10 ng/ml). |
Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. ab186671 at a concentration of 0.08 - 0.1 ug/ml yields one-half maximal inhibition [ND50] of the biological activity of Fas Ligand (10 ng/ml). |
Target
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Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
Involvement in disease
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsN-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
- Unigene: 2007 Human
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Alternative names
- ALPS1B antibody
- Apoptosis (APO 1) antigen ligand 1 antibody
- Apoptosis antigen ligand 1 antibody
see all
Datasheets and documents
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Datasheet download
References (4)
ab186671 has been referenced in 4 publications.
- Sokolova TV et al. Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy. Int J Mol Sci 23:N/A (2022). PubMed: 36293411
- Jia J et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791
- Wang JB et al. An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer. Nat Commun 11:6352 (2020). PubMed: 33311518
- Zhou Z et al. Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia-reperfusion injury via upregulation of the PI3K/Akt pathway. Mol Med Rep 18:1560-1570 (2018). PubMed: 29901074