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  1. Link

    fas-ligand-antibody-nok-1-low-endotoxin-azide-free-ab185785.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Anti-Fas Ligand antibody [NOK-1] - Low endotoxin, Azide free (ab185785)

  • Datasheet
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Key features and details

  • Mouse monoclonal [NOK-1] to Fas Ligand - Low endotoxin, Azide free
  • Suitable for: Functional Studies, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Fas Ligand antibody [NOK-1] - Low endotoxin, Azide free
    See all Fas Ligand primary antibodies
  • Description

    Mouse monoclonal [NOK-1] to Fas Ligand - Low endotoxin, Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Functional Studies, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.88% Sodium chloride
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    NOK-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Death Ligands
    • Immunology
    • Innate Immunity
    • Cytokines
    • Necrosis Factors
    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • APC Anti-Fas Ligand antibody [NOK-1] (ab210312)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human Fas Ligand protein (ab157085)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.

ab185785 has been reported in blocking of Fas Ligand mediated killing.

Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
Functional Studies
Use at an assay dependent concentration.

ab185785 has been reported in blocking of Fas Ligand mediated killing.

Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
  • Involvement in disease

    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.
  • Cellular localization

    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Target information above from: UniProt accession P48023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 356 Human
    • Omim: 134638 Human
    • SwissProt: P48023 Human
    • Unigene: 2007 Human
    • Alternative names

      • ALPS1B antibody
      • Apoptosis (APO 1) antigen ligand 1 antibody
      • Apoptosis antigen ligand 1 antibody
      • Apoptosis antigen ligand antibody
      • APT1LG1 antibody
      • APTL antibody
      • CD178 antibody
      • CD178 antigen antibody
      • CD95 ligand antibody
      • CD95-L antibody
      • CD95L antibody
      • CD95L protein antibody
      • Fas antigen ligand antibody
      • Fas L antibody
      • Fas ligand (TNF superfamily member 6) antibody
      • Fas ligand antibody
      • FASL antibody
      • Fasl Fas ligand (TNF superfamily member 6) antibody
      • FASLG antibody
      • Generalized lymphoproliferative disease antibody
      • Gld antibody
      • soluble form antibody
      • TNFL6_HUMAN antibody
      • TNFSF6 antibody
      • Tumor necrosis factor (ligand) superfamily member 6 antibody
      • Tumor necrosis factor ligand superfamily member 6 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab185785? Please let us know so that we can cite the reference in this datasheet.

    ab185785 has been referenced in 1 publication.

    • Makowska A  et al. Interferon ß and Anti-PD-1/PD-L1 Checkpoint Blockade Cooperate in NK Cell-Mediated Killing of Nasopharyngeal Carcinoma Cells. Transl Oncol 12:1237-1256 (2019). PubMed: 31295651

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