Fasciculin-II, acetylcholinesterase inhibitor (ab141801)

Overview

  • Product name

    Fasciculin-II, acetylcholinesterase inhibitor
  • Description

    Potent, reversible acetylcholinesterase inhibitor
  • Biological description

    Potent, reversible acetylcholinesterase inhibitor (Ki value = 110 pM). Toxin that causes fasciculations. Active in vitro and in vivo.
  • Purity

    > 99%
  • CAS Number

    95506-56-2
  • Chemical structure

    Chemical Structure

Properties

  • Molecular weight

    6735.00
  • Molecular formula

    C276H438N88O90S10
  • Sequence

    TMCYSHTTTSRAILTNCGENSCYRKSRRHPPKMVLGRGCGCPPGDDNLEVKCCTSPDKCNY (Modifications: Disulfide bonds: 3-22, 17-39, 41-52, 53-59)
  • Storage instructions

    Store at Room Temperature. Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water
  • Handling

    Storage after reconstitution: Up to two weeks at 4°C or six months at -20°C.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Dendroaspis angusticeps

  • Research areas

References

This product has been referenced in:

  • Bolioli B  et al. Neonatal acetylcholinesterase inhibition by fasciculin 2 in rats: a model for the study of central nervous system development? Toxicon 33:909-16 (1995). Read more (PubMed: 8588215) »
  • Fossier P  et al. Fasciculin II, a protein inhibitor of acetylcholinesterase, tested on central synapses of Aplysia. Cell Mol Neurobiol 6:221-5 (1986). Read more (PubMed: 3731216) »
  • Karlsson E  et al. Fasciculins, anticholinesterase toxins from the venom of the green mamba Dendroaspis angusticeps. J Physiol (Paris) 79:232-40 (1984). Read more (PubMed: 6530667) »

Customer reviews and Q&As

Ms.

Average
Abreviews
First of all dilute 70µg of fasciculinII with 70µl of destilated water, to have a final concentration of 1µg/µl. Using ab195225 TEXAS RED LABELLING KIT and following its protocol ( using10µl of fasciculinII) you finally have your fasciculing labelled in RED.
Then the IMUNOFLUORESCENCE PROTOCOL IN FROZEN TISSUE IS:
1. Fixe the tissue with paraformaldeid 4% 7 minutes
2. Rinse generously with PBS-Tween 0.5%-Triton 0.1% 3 times of 5 minutes each one.
3. Block the samples during 2 hours with PBS-BSA 3%
4. Rinse generously with PBS-Tween 0.5%-Triton 0.1% 3 times of 5 minutes each one
5. Incubate with labelled fasciculin II as if it was a normal antibody. Use a dilution of 1:100 or1:200 with PBS only.
6. Incubate minimum 5 hours or O/N
7. Rinse a lot and generously with PBS-Tween 0.5%-Triton 0.1% 3 times of 5 minutes each one.
8. Mount the sample with hidromount.
In the image in green Neuromuscular junction (with Alfa bungarotoxin) and in red Acetilcolinesterase.

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Verified customer

Submitted Sep 21 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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