Anti-Fascin antibody (ab183891)
Key features and details
- Rabbit polyclonal to Fascin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Fascin antibody
See all Fascin primary antibodies -
Description
Rabbit polyclonal to Fascin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Fascin aa 89-365. The exact sequence is proprietary.
Database link: Q16658 -
Positive control
- Mouse ovary tissue, HCT116 cells, HeLa whole cell lysate/extract
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the fascin family. -
Domain
Composed of four beta-trefoil domains. -
Post-translational
modificationsPhosphorylation on Ser-39 inhibits the actin-binding ability of fascin. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium. - Information by UniProt
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Database links
- Entrez Gene: 6624 Human
- Entrez Gene: 14086 Mouse
- Omim: 602689 Human
- SwissProt: Q16658 Human
- SwissProt: Q61553 Mouse
- Unigene: 118400 Human
- Unigene: 289707 Mouse
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Alternative names
- 55 kDa actin bundling protein antibody
- 55 kDa actin-bundling protein antibody
- Actin bundling protein antibody
see all
Images
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All lanes : Anti-Fascin antibody (ab183891) at 1/2000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Fascin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa -
Anti-Fascin antibody (ab183891) at 1/1000 dilution + HeLa whole cell lysate/extract at 30 µg
Predicted band size: 54 kDa -
Immunofluorescence analysis of HCT116 cells fixed in 100% methanol for 5 minutes, labeling Fascin using ab183891 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue, labeling Fascin using ab183891 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab183891 has been referenced in 4 publications.
- Gupta I et al. Oncoproteins of High-Risk HPV and EBV Cooperate to Enhance Cell Motility and Invasion of Human Breast Cancer Cells via Erk1/Erk2 and ß-Catenin Signaling Pathways. Front Oncol 11:630408 (2021). PubMed: 33777781
- Jabeen A et al. Elaeagnus angustifolia Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells. Molecules 25:N/A (2020). PubMed: 32947764
- Hua X et al. LSAMP-AS1 binds to microRNA-183-5p to suppress the progression of prostate cancer by up-regulating the tumor suppressor DCN. EBioMedicine 50:178-190 (2019). PubMed: 31727599
- Hao YJ et al. Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents. Oncol Lett 10:1333-1338 (2015). WB . PubMed: 26622672