Overview

  • Product name

    Anti-Fascin antibody [EP5902] - BSA and Azide free
    See all Fascin primary antibodies
  • Description

    Rabbit monoclonal [EP5902] to Fascin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Fascin aa 200-400. The exact sequence is proprietary.

  • General notes

    Ab240034 is the carrier-free version of ab126772. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the fascin family.
  • Domain

    Composed of four beta-trefoil domains.
  • Post-translational
    modifications

    Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
  • Information by UniProt
  • Database links

  • Alternative names

    • 55 kDa actin bundling protein antibody
    • 55 kDa actin-bundling protein antibody
    • Actin bundling protein antibody
    • actin bundling protein, 55-KD antibody
    • FAN 1 antibody
    • FAN1 antibody
    • Fascin 1 antibody
    • Fascin actin bundling protein 1 antibody
    • Fascin antibody
    • Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody
    • Fascin homolog 1 antibody
    • Fascin, sea urchin, homolog of, 1 antibody
    • Fascin1 antibody
    • FLJ38511 antibody
    • FSCN 1 antibody
    • FSCN1 antibody
    • FSCN1_HUMAN antibody
    • HSN antibody
    • p55 antibody
    • Singed (Drosophila) like (sea urchin fascin homolog like) antibody
    • Singed drosophila homolog like antibody
    • Singed like (fascin homolog sea urchin) (Drosophila) antibody
    • Singed like (fascin homolog sea urchin) antibody
    • Singed like protein antibody
    • Singed, drosophila, homolog of antibody
    • Singed-like protein antibody
    • SNL antibody
    • Strongylocentrotus purpuratus antibody
    see all

Images

  • ab126772, at 1/250 dilution, staining Fascin in paraffin-embedded Human Hodgkin's lymphoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) labelling Fascin with purified ab126772 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

  • Overlay histogram showing HeLa cells stained with ab126772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126772, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

  • ab126772, at 1/250 dilution, staining Fascin in paraffin-embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab126772 showing positive staining in Normal tonsil tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab126772 showing positive staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab126772 showing positive staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126772).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab240034 has not yet been referenced specifically in any publications.

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