Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2681] to Fascin
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-Fascin antibody [EPR2681]
See all Fascin primary antibodies
DescriptionRabbit monoclonal [EPR2681] to Fascin
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Fascin aa 400-500 (internal sequence). The exact sequence is proprietary.
- HeLa, SH-SY5Y and K-562 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.42 x 10 -9 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 55 kDa.|
|Flow Cyt||1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionOrganizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
Sequence similaritiesBelongs to the fascin family.
DomainComposed of four beta-trefoil domains.
modificationsPhosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
- Information by UniProt
- 55 kDa actin bundling protein antibody
- 55 kDa actin-bundling protein antibody
- Actin bundling protein antibody
All lanes : Anti-Fascin antibody [EPR2681] (ab108607) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab108607 has not yet been referenced specifically in any publications.