Anti-Fascin antibody [FSCN1/417] (ab220195)
Key features and details
- Mouse monoclonal [FSCN1/417] to Fascin
- Suitable for: WB, ICC, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Fascin antibody [FSCN1/417]
See all Fascin primary antibodies -
Description
Mouse monoclonal [FSCN1/417] to Fascin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Fascin aa 1 to the C-terminus.
Database link: Q16658 -
Positive control
- Human Hodgkin's lymphoma, ovarian carcinoma and testicular carcinoma tissues; HeLa and K562 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab220195 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
FSCN1/417 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 54 kDa.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use 1-2µg for 106 cells.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 54 kDa. |
ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Function
Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the fascin family. -
Domain
Composed of four beta-trefoil domains. -
Post-translational
modificationsPhosphorylation on Ser-39 inhibits the actin-binding ability of fascin. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium. - Information by UniProt
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Database links
- Entrez Gene: 6624 Human
- Entrez Gene: 14086 Mouse
- Entrez Gene: 683788 Rat
- Omim: 602689 Human
- SwissProt: Q16658 Human
- SwissProt: Q61553 Mouse
- SwissProt: P85845 Rat
- Unigene: 118400 Human
see all -
Alternative names
- 55 kDa actin bundling protein antibody
- 55 kDa actin-bundling protein antibody
- Actin bundling protein antibody
see all
Images
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Flow cytometric analysis of PFA-fixed K562 cells using ab220195 followed by a Goat anti-Mouse IgG (blue); isotype control (red)
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mmunofluorescence Analysis of HeLa cells labeling Fascin with ab220195 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red)
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Anti-Fascin antibody [FSCN1/417] (ab220195) at 1 µg/ml + HeLa cell lysate
Predicted band size: 54 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Hodgkin's lymphoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab220195 has been referenced in 3 publications.
- Wu Y et al. LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism. Oxid Med Cell Longev 2021:9979707 (2021). PubMed: 35069968
- He S et al. MicroRNA-145-5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells. Mol Med Rep 22:5282-5292 (2020). PubMed: 33174029
- Chen Z et al. MicroRNA-145 regulates immune cytokines via targeting FSCN1 in Staphylococcus aureus-induced mastitis in dairy cows. Reprod Domest Anim 54:882-891 (2019). PubMed: 30974481