Overview

  • Product name

    Anti-Fascin antibody [SPM133] - BSA and Azide free
    See all Fascin primary antibodies
  • Description

    Mouse monoclonal [SPM133] to Fascin - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Human Fascin aa 2-493.
    Sequence:

    TANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQP PDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRW SLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKR YAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGR LVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKD ELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRD TKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASN GKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVT GTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDF FFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY


    Database link: Q16658

  • Positive control

    • Human Hodgkin's lymphoma,ovarian and testicular carcinoma tissues; HeLa, MCF-7, PC-3 and BEWO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab212841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the fascin family.
  • Domain

    Composed of four beta-trefoil domains.
  • Post-translational
    modifications

    Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
  • Information by UniProt
  • Database links

  • Alternative names

    • 55 kDa actin bundling protein antibody
    • 55 kDa actin-bundling protein antibody
    • Actin bundling protein antibody
    • actin bundling protein, 55-KD antibody
    • FAN 1 antibody
    • FAN1 antibody
    • Fascin 1 antibody
    • Fascin actin bundling protein 1 antibody
    • Fascin antibody
    • Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody
    • Fascin homolog 1 antibody
    • Fascin, sea urchin, homolog of, 1 antibody
    • Fascin1 antibody
    • FLJ38511 antibody
    • FSCN 1 antibody
    • FSCN1 antibody
    • FSCN1_HUMAN antibody
    • HSN antibody
    • p55 antibody
    • Singed (Drosophila) like (sea urchin fascin homolog like) antibody
    • Singed drosophila homolog like antibody
    • Singed like (fascin homolog sea urchin) (Drosophila) antibody
    • Singed like (fascin homolog sea urchin) antibody
    • Singed like protein antibody
    • Singed, drosophila, homolog of antibody
    • Singed-like protein antibody
    • SNL antibody
    • Strongylocentrotus purpuratus antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue labeling Fascin with ab212841 at 1 µg/mL.

References

ab212841 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab212841.
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