Key features and details
- Mouse monoclonal [SPM133] to Fascin - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Fascin antibody [SPM133] - BSA and Azide free
See all Fascin primary antibodies
DescriptionMouse monoclonal [SPM133] to Fascin - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant full length protein corresponding to Human Fascin aa 2-493.
TANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQP PDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRW SLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKR YAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGR LVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKD ELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRD TKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASN GKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVT GTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDF FFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Database link: Q16658
- Human Hodgkin's lymphoma,ovarian and testicular carcinoma tissues; HeLa, MCF-7, PC-3 and BEWO cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab212841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
(in 0.1 ml).
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
FunctionOrganizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
Sequence similaritiesBelongs to the fascin family.
DomainComposed of four beta-trefoil domains.
modificationsPhosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium.
- Information by UniProt
- 55 kDa actin bundling protein antibody
- 55 kDa actin-bundling protein antibody
- Actin bundling protein antibody
ab212841 has not yet been referenced specifically in any publications.