Key features and details
- Tissue and protein stain.
- CAS Number: 2353-45-9
- Soluble in water to 1mg/ml
- Form / State: Solid
- Source: Synthetic
Product nameFast green FCF, tissue and protein stain
DescriptionTissue and protein stain.
- F D and C Green No. 3
- FD&C Green No. 3
Widely utilized tissue and protein stain. Used as a quantitative stain for histones at alkaline pH after acid extraction of DNA and as a protein stain in IEF (isoelectric focusing) and SDS-PAGE electrophoresis. Fluoresces in the near-infrared (NIR). Recommended as a replacement for Light Green SF yellowish in Masson's trichrome as its color is more brilliant and less likely to fade. Also used as a food dye.
Chemical nameN-Ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](4-hydroxy-2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium inner salt disodium salt
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 1mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab146267 has not yet been referenced specifically in any publications.