Product nameFast green FCF, tissue and protein stain
DescriptionTissue and protein stain.
- F D and C Green No. 3
- FD&C Green No. 3
Widely utilized tissue and protein stain. Used as a quantitative stain for histones at alkaline pH after acid extraction of DNA and as a protein stain in IEF (isoelectric focusing) and SDS-PAGE electrophoresis. Fluoresces in the near-infrared (NIR). Recommended as a replacement for Light Green SF yellowish in Masson's trichrome as its color is more brilliant and less likely to fade. Also used as a food dye.
Chemical nameN-Ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](4-hydroxy-2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium inner salt disodium salt
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 1mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Elliott DG et al. Evaluation of fast green FCF dye for non-lethal detection of integumental injuries in juvenile Chinook salmon Oncorhynchus tshawytscha. Dis Aquat Organ 84:139-50 (2009). Read more (PubMed: 19476284) »
- Luo S et al. Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green. Anal Biochem 350:233-8 (2006). Read more (PubMed: 16336940) »
- van Hooft JA Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. Neurosci Lett 318:163-5 (2002). Read more (PubMed: 11803124) »
- Shen HD et al. An improved scheme for the identification of antigens recognized by specific antibodies in two-dimensional gel electrophoresis and immunoblotting. Electrophoresis 11:878-82 (1990). Read more (PubMed: 2079030) »