Recombinant
RabMAb

Recombinant Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149)

Overview

  • Product name

    Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64]
  • Description

    Rabbit monoclonal [EPR22880-64] to Fast Myosin Skeletal Heavy chain + MYH4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Fast Myosin Skeletal Heavy chain aa 800-1200. The exact sequence is proprietary. (SwissProt ID Q9Y623).
    Database link: P12882

  • Positive control

    • WB: Human skeletal muscle tissue lysate. IHC-P: Human skeletal muscle tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 223 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.
  • Target

    Images

    • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human skeletal muscle (PMID: 30274168, 25217814).

      The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human liver (PMID: 30274168). The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

    • Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149) at 1/1000 dilution + Human skeletal muscle tissue lysate at 20 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 223 kDa


      Exposure time: 37 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    References

    ab221149 has not yet been referenced specifically in any publications.

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