Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506)
Key features and details
- Rabbit polyclonal to Fast Myosin Skeletal Heavy chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Fast Myosin Skeletal Heavy chain antibody
See all Fast Myosin Skeletal Heavy chain primary antibodies -
Description
Rabbit polyclonal to Fast Myosin Skeletal Heavy chain -
Host species
Rabbit -
Specificity
Replenishment batches of ab91506 are tested in IHC-P. Previous batches were additionally validated in WB. This application is still expected to work and is covered by our Abpromise guarantee.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Guinea pig, Cow, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab102066) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).
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IHC-P | (1) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
Target
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Function
Muscle contraction. -
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
Cellular localization
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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Database links
- Entrez Gene: 281337 Cow
- Entrez Gene: 4619 Human
- Entrez Gene: 17879 Mouse
- Entrez Gene: 287408 Rat
- Omim: 160730 Human
- Omim: 160740 Human
- SwissProt: Q9BE40 Cow
- SwissProt: P12882 Human
see all -
Alternative names
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
Images
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All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Formalin-fixed, paraffin-embedded pig skeletal muscle tissue stained for Fast Myosin Skeletal Heavy chain using ab91506 at 1/1000 dilution (for 2 hours at room temperature) in immunohistochemical analysis (upper image).
Note: Lower image is a comparison using ab51263.
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IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (63)
ab91506 has been referenced in 63 publications.
- Wen W et al. Dietary lycopene supplementation improves meat quality, antioxidant capacity and skeletal muscle fiber type transformation in finishing pigs. Anim Nutr 8:256-264 (2022). PubMed: 34988307
- Kawao N et al. Renal failure suppresses muscle irisin expression, and irisin blunts cortical bone loss in mice. J Cachexia Sarcopenia Muscle 13:758-771 (2022). PubMed: 34997830
- Cai A et al. Microsurgical Transplantation of Pedicled Muscles in an Isolation Chamber-A Novel Approach to Engineering Muscle Constructs via Perfusion-Decellularization. J Pers Med 12:N/A (2022). PubMed: 35330443
- Fortino SA et al. Sex-Based Differences in the Myogenic Response and Inflammatory Gene Expression Following Eccentric Contractions in Humans. Front Physiol 13:880625 (2022). PubMed: 35574443
- Wang M et al. Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues. Nat Commun 13:3317 (2022). PubMed: 35680907