Product nameAnti-Fast Myosin Skeletal Heavy chain antibody
See all Fast Myosin Skeletal Heavy chain primary antibodies
DescriptionRabbit polyclonal to Fast Myosin Skeletal Heavy chain
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Sheep, Guinea pig, Cow
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Fast Myosin Skeletal Heavy chain.
- This antibody gave a positive signal in the following lysates: Skeletal Muscle (Human) Tissue Lysate; Skeletal Muscle (Mouse) Tissue Lysate; Skeletal Muscle (Rat) Tissue Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab91506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23515448|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 223 kDa (predicted molecular weight: 223 kDa).|
|IHC-P||Use a concentration of 1 µg/ml.|
Sequence similaritiesContains 1 IQ domain.
Contains 1 myosin head-like domain.
DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
- Information by UniProt
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
IHC image of Fast Myosin Skeletal Heavy chain staining in Mouse skeletal muscle FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
All lanes : Anti-Fast Myosin Skeletal Heavy chain antibody (ab91506) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab91506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Immunohistochemical analysis of sheep muscle tissue frozen section, labeling Fast Myosin Skeletal Heavy Chain with ab91506. Samples were fixed in methanol, blocking was with 3% BSA for 30 minutes at 25°C. Samples were incubated with ab91506 diluted 1/200 for 16 hours at 4°C.
This product has been referenced in:
- Siemionow M et al. Dystrophin Expressing Chimeric (DEC) Human Cells Provide a Potential Therapy for Duchenne Muscular Dystrophy. Stem Cell Rev 14:370-384 (2018). Read more (PubMed: 29546607) »
- Takano Y et al. Fat infiltration in the gluteus minimus muscle in older adults. Clin Interv Aging 13:1011-1017 (2018). Read more (PubMed: 29872279) »