We also try to use 6% and 12% Gel.
We try to block the membrane in 3% BSA in TBST and to use 1:2000 primery antibody concentration. All failed.
Thank you for your review of this previously untested species, Bos taurus. Further troubleshooting did not improve the staining pattern observed. Abcam would welcome feedback from other customers. Please contact us to participate in our Abtrial program: www.abcam.com/abtrial.
Dr. Osnat Ophir
Submitted Oct 02 2017
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