Western blot abreview for Anti-Fast Myosin Skeletal Heavy chain antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Bos taurus Tissue lysate - other (muscle)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
10 µg
Specification
muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/50000
Name

Detection

Detection method
ECL
Exposure
10 second(s)
Positive control
My Sample

Additional data

Additional Notes
WB: We also try to use 6% and 12% Gel. We try to block the membrane in 3% BSA in TBST and to use 1:2000 primery antibody concentration. All failed.
Abcam response
Thank you for your review of this previously untested species, Bos taurus. Further troubleshooting did not improve the staining pattern observed. Abcam would welcome feedback from other customers. Please contact us to participate in our Abtrial program: www.abcam.com/abtrial.

Dr. Osnat Ophir

Verified customer

Submitted Oct 02 2017

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