Immunocytochemistry/ Immunofluorescence abreview for Anti-Fast Myosin Skeletal Heavy chain antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skeletal muscle, differentiated myoblasts)
Permeabilization
Yes - 0.5% Triton X100
Specification
Skeletal muscle, differentiated myoblasts
Blocking step
10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% goat serum
Dilution
1/250

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Skeletal muscle cells cultured on plain contained a mixed population of skeletal myoblasts and fibroblasts.
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the use of ab91506 for ICC/IF, an application we have not tested before.

Mrs. Hanis Hasmad

Verified customer

Submitted Apr 09 2018

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