Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fast Myosin Skeletal Heavy chain antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/1000

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted composite image (upper image ab91506, lower image ab51263) of sequential sections shows identical positivity. Section quality is variable, however. This is the 1st Rabbit anti Myosin heavy chain (fast/slow fibre) that I have come across that is successful in IHC-P.

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2018

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