Overview

  • Product name
    Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]
    See all Fast Myosin Skeletal Heavy chain primary antibodies
  • Description
    Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.

  • Positive control
    • Human skeletal muscle
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 (GR255796). Lot numbers higher than GR255796 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    May be used for detecting cross striated muscle differentiation in tumors.

Properties

Applications

Our Abpromise guarantee covers the use of ab51263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 223 kDa.
IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.

Use at an assay dependent concentration.

Target

  • Function
    Muscle contraction.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin-1 antibody
    • skeletal muscle antibody
    see all

Images

  • ab51263 staining Fast Myosin Skeletal Heavy chain in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab51263 staining Fast Myosin Skeletal Heavy chain in pig skeletal muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Song M  et al. Trimetazidine restores the positive adaptation to exercise training by mitigating statin-induced skeletal muscle injury. J Cachexia Sarcopenia Muscle 9:106-118 (2018). IF . Read more (PubMed: 29152896) »
  • Mattyasovszky SG  et al. Exposure to radial extracorporeal shock waves modulates viability and gene expression of human skeletal muscle cells: a controlled in vitro study. J Orthop Surg Res 13:75 (2018). Read more (PubMed: 29625618) »
See all 17 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2018

Answer

I can confirm that ab51263 Fast Myosin Skeletal Heavy chain antibody [MY-32] was produced from spleen of mouse immunised with rabbit muscle myosin. I am sorry that regrettably no other specification as to its epitope identity has been assessed. Nevertheless, this antibody has been used in many papers (a few of them are cited below), which I hope may be useful to you:


PNAS 86; 7007-7011 (1989)

Development 107; 751-769 (1989)

Histochemistry 93; 497-499 (1990)

Histopathology 17; 301-309 (1990)

Differentiation 55; 203-211 (1994)

I am sorry we do not have the specific information you require on this occasion, but I hope this will be helpful.

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Answer

r these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge in order number#

Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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Question

Product code: 51263

Inquiry: 1) Order details: ˙Batch number (Lot number): gr25963-2 Po: 1069725 ˙Abcam product code: ab51263 ˙Antibody storage conditions (temperature/reconstitution etc) : -20℃
2) Please describe the problem (high background, non-specific signal…etc). It can work with ab11083, but non-specific signal with ab51263 (same condition and same muscle).
3) On what material are you testing the antibody in IHC/ICC? ˙Species: mice (C57/BL6) ˙What’s cell line or tissue: skeletal muscle
4) Sample preparation: ˙Type of sample: formalin fixed ˙paraffin embedded sections, cells in culture, other:____) : paraffin embedded sections ˙Sample preparation: ˙Positive control : ˙Negative control :
5) Fixation step ˙Yes or No : Yes ˙If yes, Fixative agent and concentration: 10 % neutral buffer formalin ˙Fixation time: ˙Fixation temperature: room temperature
6) Antigen retrieval method(including time, temperature etc.): heat 15 mins
7) Permeabilization method: ˙Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? ˙Permeabilizing agent and concentration:
8) Blocking agent (eg BSA, serum…): ˙Concentration: 3% ˙Blocking time :2 hr ˙Blocking temperature: room temperature
9) ˙Endogenous peroxidases blocked? ˙Endogenous biotins blocked?
10) Primary antibody (If more than one was used, describe in “additional notes”) : ˙Species: mice ˙Reacts against: ˙At what dilution(s) have you tested this antibody:1:100 and 1:150 ˙Diluent buffer: 1% BSA ˙Incubation time: overnight ˙Incubation temperature: 4℃ ˙What washing steps were done (which buffer, number of washes): TBST, 3×10 mins
11) Secondary antibody: supersensitivy polymer-HRP IHC detecting system, QD4200810(Lot number) ˙Species: ˙Reacts against which species: ˙At what dilution(s) have you tested this antibody: ˙Diluent buffer: ˙Incubation time: ˙What washing steps were done (which buffer, number of washes): ˙Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): ˙Do you know whether the problems you are experiencing come from the secondary?
12) Signal amplification method (eg: ABC, LSAB, HRP polymer, TSE): HRP polymer
13) Detection method (eg: DAB, BCIP/NBT …etc):DAB
14) ˙ How many times have you run this staining? 3 times ˙Do you obtain the same results every time? YES ˙What steps have you altered to try and optimize the use of this antibody? antibody dilution(s)¸1:150 to 1:100

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Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab51263 Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]. I would also appreciate if you can confirm some further details:

1. Please keep in mind that you cannot compare two different antibodies against two different targets with each other. Every antibody needs its individual optimization.

2. What was the HIER buffer? Did the customer perform a time series to find the optimal point? I would strongly recommend to perform a time series of at least 3 different retrieval times (like 15, 20, 25 min) to find the optimal time. As every antibody is different, every epitope may show a different retrieval time. The occurrence ofno signalusually shows a too short retrieval.

3. Isthe customer using a detergent like Tween or Triton during incubation? If not, I can recommend adding Triton to your incubation solution to facilitate the antibodies solubility, but in a low concentration of 0.025%.

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

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Question
Answer

Apologies for the delay, I was waiting for further information.

Our products team has confirmed that these products have the correct alternative names listed. As stated on the datasheets they will specifically stain either the fast or slow fibers.

I hope this information is useful.

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Answer

According to our records, ab51263 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to a reply.  

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Answer

Thank you for contacting us. We have following products available in catalogue that are best match as per your request. ab11083 ab51263 ab104128 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

r.

I am sorry that I couldn't help the customer on this occasion.


I have checked the complaint rate for this antibody, and we had only 9complaints in over 2 years with over 150 units sold. therefore, i think this is usually a very reliable product, but your customer unfortunately received a faulty vial.

I will gladly send them a replacement or a credit note, whatever they prefer.

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