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    fast-red-substrate-kit-ab64254.pdf

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Fast Red Substrate Kit (ab64254)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (6)

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IHC of human esophageal carcinoma
  • IHC of human tonsil

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Overview

  • Product name

    Fast Red Substrate Kit
  • Product overview

    Fast Red Substrate kit ab64254 for immunohistochemical staining using alkaline phosphatase-based detection.


    Fast Red can be used as chromogen for immunohistochemical staining. In the presence of alkaline phosphatase enzyme, Fast Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Notes

    This product was previously called Liquid Fast Red Substrate Kit.

    Streptavidin - Alkaline phospatase ab246833 is suitable for use with Fast red substrate.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-P, WBmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Storage buffer

    pH: 8.50
    Constituents: 20% Fast Red TR salt, 20% Naphthol AS-MX phosphate, Tris buffer
  • Components 125 ml 60 ml
    75x Fast-Red Chromogen 1 x 2ml 1 x 1ml
    Liquid Fast Red Substrate 1 x 125ml 1 x 60ml
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Substrate
  • Relevance

    Fast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab64254 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution.
    Notes
    IHC-P
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution.

    Images

    • IHC of human esophageal carcinoma
      IHC of human esophageal carcinoma

      Immunohistochemical staining of human esophageal carcinoma with ab40084 at a dilution of 1/200 and detected using ab64254.

    • IHC of human tonsil
      IHC of human tonsil

      Immunohistochemical staining of human tonsil with ab16667 at a dilution of 1/200 and detected using ab64254.

    Protocols

    • Liquid Fast-Red Substrate Kit
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab64254? Please let us know so that we can cite the reference in this datasheet.

    ab64254 has been referenced in 6 publications.

    • Dominguez G  et al. Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain. Acta Neuropathol Commun 6:25 (2018). PubMed: 29615132
    • Schmidt L  et al. Increased FGF8 signaling promotes chondrogenic rather than osteogenic development in the embryonic skull. Dis Model Mech 11:N/A (2018). PubMed: 29752281
    • Zhang LL  et al. Interference with lactate metabolism by mmu-miR-320-3p via negatively regulating GLUT3 signaling in mouse Sertoli cells. Cell Death Dis 9:964 (2018). PubMed: 30237478
    • Shang H  et al. Effects of maternal dexamethasone treatment early in pregnancy on glucocorticoid receptors in the ovine placenta. Reprod Sci 22:534-44 (2015). PubMed: 25332218
    • Ceccom J  et al. Reduced sphingosine kinase-1 and enhanced sphingosine 1-phosphate lyase expression demonstrate deregulated sphingosine 1-phosphate signaling in Alzheimer's disease. Acta Neuropathol Commun 2:12 (2014). IHC-P . PubMed: 24468113
    • Srivatsa S  et al. Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation. Nat Commun 5:3708 (2014). IHC-Fr . PubMed: 24739528

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Substrateis not giving any staining, but previous kits have worked well.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 15 2012

    Answer

    Thank you for your call today and for letting us know about the trouble with ab64254.

    I have issued credit note# ***, and the full purchase price of ab64524will be refunded to your credit card pending accounts approval. If youhave any questions about this credit, please email mailto:us.credits@abcam.com and reference the credit note number.

    Please let me know if you have any questions, and keep me updated about the results with the newsubstrate that youordered today. I look forward to hearing from you.Have a great day!

    Read More

    Abcam Scientific Support

    Answered on Mar 15 2012

    Question

    There is a yellow staining after 10 minutes of incubation. No red color.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 29 2012

    Answer

    Thanks for your call today and for letting us know about the trouble with this kit.

    I'm wondering if you've had a chance to try a longer incubation with the reagent? What were the results? Please feel free to give us a call back if you prefer, or reply to this email, and if the problem has persisted then I will be happy to get in touch with the lab to enquire about the cause of this.

    I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Feb 29 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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