Product nameFast Red Substrate Kit
Fast Red Substrate kit ab64254 for immunohistochemical staining using alkaline phosphatase-based detection.
Fast Red can be used as chromogen for immunohistochemical staining. In the presence of alkaline phosphatase enzyme, Fast Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.
This product was previously called Liquid Fast Red Substrate Kit.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Tested applicationsSuitable for: IHC-P, WBmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Storage bufferpH: 8.50
Constituents: 20% Fast Red TR salt, 20% Naphthol AS-MX phosphate, Tris buffer
Components 125 ml 60 ml 125 ml 75x Fast-Red Chromogen 1 x 2ml 1 x 1ml 1 x 2ml Liquid Fast Red Substrate 1 x 125ml 1 x 60ml 1 x 125ml
RelevanceFast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.
Our Abpromise guarantee covers the use of ab64254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
This product has been referenced in:
- Dominguez G et al. Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain. Acta Neuropathol Commun 6:25 (2018). Read more (PubMed: 29615132) »
- Schmidt L et al. Increased FGF8 signaling promotes chondrogenic rather than osteogenic development in the embryonic skull. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29752281) »