Fast Red Substrate Kit (ab64254)

Submit a review Q&A (2)References (6)

Overview

  • Product name

    Fast Red Substrate Kit

  • Product overview

    Fast Red Substrate kit ab64254 for immunohistochemical staining using alkaline phosphatase-based detection.


    Fast Red can be used as chromogen for immunohistochemical staining. In the presence of alkaline phosphatase enzyme, Fast Red produces a red reaction product that can be seen using either brightfield or fluorescent microscopy.

  • Notes

    This product was previously called Liquid Fast Red Substrate Kit.

    Streptavidin - Alkaline phospatase ab246833 is suitable for use with Fast red substrate.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Tested applications

    Suitable for: IHC-P, WBmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Storage buffer

    pH: 8.50
    Constituents: 20% Fast Red TR salt, 20% Naphthol AS-MX phosphate, Tris buffer
  • Components 125 ml 60 ml
    75x Fast-Red Chromogen 1 x 2ml 1 x 1ml
    Liquid Fast Red Substrate 1 x 125ml 1 x 60ml

  • Research areas

  • Relevance

    Fast-Red is a widely used chromogen which produces a red reaction product in the presence of alkaline phosphatase enzyme.

Applications

Our Abpromise guarantee covers the use of ab64254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Images

  • IHC of human esophageal carcinoma
    IHC of human esophageal carcinoma

    Immunohistochemical staining of human esophageal carcinoma with ab40084 at a dilution of 1/200 and detected using ab64254.

  • IHC of human tonsil
    IHC of human tonsil

    Immunohistochemical staining of human tonsil with ab16667 at a dilution of 1/200 and detected using ab64254.

References

This product has been referenced in:

  • Dominguez G  et al. Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain. Acta Neuropathol Commun 6:25 (2018). Read more (PubMed: 29615132) »
  • Schmidt L  et al. Increased FGF8 signaling promotes chondrogenic rather than osteogenic development in the embryonic skull. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29752281) »
See all 6 Publications for this product

Publishing research using ab64254? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Substrateis not giving any staining, but previous kits have worked well.

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Answer

Thank you for your call today and for letting us know about the trouble with ab64254.

I have issued credit note# ***, and the full purchase price of ab64524will be refunded to your credit card pending accounts approval. If youhave any questions about this credit, please email mailto:us.credits@abcam.com and reference the credit note number.

Please let me know if you have any questions, and keep me updated about the results with the newsubstrate that youordered today. I look forward to hearing from you.Have a great day!

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Question

There is a yellow staining after 10 minutes of incubation. No red color.

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Answer

Thanks for your call today and for letting us know about the trouble with this kit.

I'm wondering if you've had a chance to try a longer incubation with the reagent? What were the results? Please feel free to give us a call back if you prefer, or reply to this email, and if the problem has persisted then I will be happy to get in touch with the lab to enquire about the cause of this.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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