Overview

  • Product name

    Anti-fast skeletal Myosin antibody
    See all fast skeletal Myosin primary antibodies
  • Description

    Rabbit polyclonal to fast skeletal Myosin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Pig
  • Immunogen

    Recombinant fragment within Human fast skeletal Myosin (internal sequence). The exact sequence is proprietary.
    Database link: P05976

  • Positive control

    • WB: HeLa whole cell lysate; Mouse heart lysate; Rat muscle lysate. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.

Target

Images

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for fast skeletal Myosin (green) using ab228727 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-fast skeletal Myosin antibody (ab228727) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 21 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution

    Lane 1 : Mouse heart lysate at 1 µg
    Lane 2 : Mouse heart lysate at 5 µg

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 21 kDa



    15% SDS-PAGE gel.

  • Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution + Rat muscle lysate at 1 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 21 kDa



    15% SDS-PAGE gel.

References

ab228727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228727.
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