Anti-fast skeletal Myosin antibody (ab228727)
Key features and details
- Rabbit polyclonal to fast skeletal Myosin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-fast skeletal Myosin antibody -
Description
Rabbit polyclonal to fast skeletal Myosin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Pig -
Immunogen
Recombinant fragment within Human fast skeletal Myosin (internal sequence). The exact sequence is proprietary.
Database link: P05976 -
Positive control
- WB: HeLa whole cell lysate; Mouse heart lysate; Rat muscle lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
Target
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Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 317657 Cow
- Entrez Gene: 29895 Human
- Entrez Gene: 4632 Human
- Entrez Gene: 17901 Mouse
- Entrez Gene: 17907 Mouse
- Entrez Gene: 397666 Pig
- Entrez Gene: 24584 Rat
- Entrez Gene: 56781 Rat
see all -
Alternative names
- A1 catalytic antibody
- A2 catalytic antibody
- Alkali myosin light chain 1 antibody
see all
Images
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for fast skeletal Myosin (green) using ab228727 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Anti-fast skeletal Myosin antibody (ab228727) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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All lanes : Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution
Lane 1 : Mouse heart lysate at 1 µg
Lane 2 : Mouse heart lysate at 5 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution + Rat muscle lysate at 1 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab228727 has been referenced in 2 publications.
- Qian Y et al. Preclinical assessment on neuronal regeneration in the injury-related microenvironment of graphene-based scaffolds. NPJ Regen Med 6:31 (2021). PubMed: 34078912
- DeChick A et al. Increased Skeletal Muscle Fiber Cross-Sectional Area, Muscle Phenotype Shift, and Altered Insulin Signaling in Rat Hindlimb Muscles in a Prenatally Androgenized Rat Model for Polycystic Ovary Syndrome. Int J Mol Sci 21:N/A (2020). PubMed: 33113794