Overview

  • Product name
    Anti-FAT10 antibody [EPR4370]
    See all FAT10 primary antibodies
  • Description
    Rabbit monoclonal [EPR4370] to FAT10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    within Human FAT10 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O15205

  • Positive control
    • HepG2, MOLT4, K562, and Daudi cell lysates; Human kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).
    • Tissue specificity
      Constitutively expressed in mature dendritic cells and B cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.
    • Sequence similarities
      Contains 2 ubiquitin-like domains.
    • Post-translational
      modifications
      Can be acetylated.
    • Cellular localization
      Nucleus. Cytoplasm. Accumulates in aggresomes under proteasome inhibition conditions.
    • Information by UniProt
    • Database links
    • Alternative names
      • Diubiquitin antibody
      • FAT10 antibody
      • GABBR1 antibody
      • UBD 3 antibody
      • Ubd antibody
      • UBD_HUMAN antibody
      • Ubiquitin D antibody
      • Ubiquitin like protein FAT10 antibody
      • Ubiquitin-like protein FAT10 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling FAT10 with ab134077 at a dilution of 1/50.

    • All lanes : Anti-FAT10 antibody [EPR4370] (ab134077) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa

    References

    This product has been referenced in:
    • Li C  et al. Human HLA-F adjacent transcript 10 promotes the formation of cancer initiating cells and cisplatin resistance in bladder cancer. Mol Med Rep 18:308-314 (2018). Read more (PubMed: 29749526) »
    • Ge Q  et al. Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis. Front Physiol 9:1051 (2018). Read more (PubMed: 30131712) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 100C, 20 min
    Permeabilization
    No
    Specification
    kidney
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 06 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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