Product nameAnti-FAT10 antibody [EPR4370]
See all FAT10 primary antibodies
DescriptionRabbit monoclonal [EPR4370] to FAT10
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Human
within Human FAT10 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: O15205
- HepG2, MOLT4, K562, and Daudi cell lysates; Human kidney tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
FunctionUbiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).
Tissue specificityConstitutively expressed in mature dendritic cells and B cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.
Sequence similaritiesContains 2 ubiquitin-like domains.
modificationsCan be acetylated.
Cellular localizationNucleus. Cytoplasm. Accumulates in aggresomes under proteasome inhibition conditions.
- Information by UniProt
- Diubiquitin antibody
- FAT10 antibody
- GABBR1 antibody
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling FAT10 with ab134077 at a dilution of 1/50.
All lanes : Anti-FAT10 antibody [EPR4370] (ab134077) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
This product has been referenced in:
- Li C et al. Human HLA-F adjacent transcript 10 promotes the formation of cancer initiating cells and cisplatin resistance in bladder cancer. Mol Med Rep 18:308-314 (2018). Read more (PubMed: 29749526) »
- Ge Q et al. Mulberry Leaf Regulates Differentially Expressed Genes in Diabetic Mice Liver Based on RNA-Seq Analysis. Front Physiol 9:1051 (2018). Read more (PubMed: 30131712) »