Recombinant
RabMAb

Recombinant Anti-FAT10 antibody [EPR4370] - BSA and Azide free (ab246336)

Overview

  • Product name
    Anti-FAT10 antibody [EPR4370] - BSA and Azide free
    See all FAT10 primary antibodies
  • Description
    Rabbit monoclonal [EPR4370] to FAT10 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human FAT10 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O15205

  • Positive control
    • HepG2, MOLT4, K562, and Daudi cell lysates; Human kidney tissue.
  • General notes

    ab246336 is a PBS-only buffer format of ab134077. Please refer to ab134077 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab246336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN).
    • Tissue specificity
      Constitutively expressed in mature dendritic cells and B cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.
    • Sequence similarities
      Contains 2 ubiquitin-like domains.
    • Post-translational
      modifications
      Can be acetylated.
    • Cellular localization
      Nucleus. Cytoplasm. Accumulates in aggresomes under proteasome inhibition conditions.
    • Information by UniProt
    • Database links
    • Alternative names
      • Diubiquitin antibody
      • FAT10 antibody
      • GABBR1 antibody
      • UBD 3 antibody
      • Ubd antibody
      • UBD_HUMAN antibody
      • Ubiquitin D antibody
      • Ubiquitin like protein FAT10 antibody
      • Ubiquitin-like protein FAT10 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling FAT10 with ab134077 at a dilution of 1/50.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134077).

    References

    ab246336 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab246336.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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