Anti-FAT4 antibody (ab130076)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-FAT4 antibody

  • Description

    Rabbit polyclonal to FAT4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide conjugated to KLH corresponding to a region within Human FAT4 (SwissProt Q6V0I7).

  • Positive control

    • recombinant FAT4 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab130076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 543 kDa).
ICC/IF 1/50 - 1/100.

Target

  • Function

    May function in the regulation of planar cell polarity (By similarity). Cadherins are cell-cell interaction molecules.

  • Tissue specificity

    Widely expressed. Expressed in fetal brain, infant brain, brain tumor and colorectal cancer.

  • Sequence similarities

    Contains 34 cadherin domains.
    Contains 6 EGF-like domains.
    Contains 2 laminin G-like domains.

  • Cellular localization

    Membrane. In the kidney, localizes to primary cilia.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cadherin family member 14 antibody
      • Cadherin related family member 11 antibody
      • CDHF14 antibody
      • CDHR11 antibody
      • FAT atypical cadherin 4 antibody
      • FAT J antibody
      • FAT tumor suppressor homolog 4 antibody
      • Fat-like cadherin protein FAT-J antibody
      • FAT4 antibody
      • FAT4_HUMAN antibody
      • FATJ antibody
      • hFat4 antibody
      • HKLLS2 antibody
      • Nbla00548 antibody
      • Protocadherin Fat 4 antibody
      • Putative protein product of Nbla00548 antibody
      • VMLDS2 antibody
      see all

    Images

    • Western blot - Anti-FAT4 antibody (ab130076)
      Western blot - Anti-FAT4 antibody (ab130076)
      Anti-FAT4 antibody (ab130076) at 1/500 dilution + recombinant FAT4 protein

      Predicted band size: 543 kDa



      actual MW: 48kDa

    References

    This product has been referenced in:

    • Che X  et al. FAT4-USP51 complex regulates the proliferation and invasion of endometrial cancer via Hippo pathway. Am J Transl Res 11:2784-2800 (2019). Read more (PubMed: 31217854) »
    • Li SY  et al. Down-regulated long non-coding RNA RNAZFHX4-AS1 suppresses invasion and migration of breast cancer cells via FAT4-dependent Hippo signaling pathway. Cancer Gene Ther N/A:N/A (2018). Read more (PubMed: 30546116) »
    See all 3 Publications for this product

    Publishing research using ab130076? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Where is endogenous FAT4 expected to appear in an SDS-PAGE gel/western blot? What is the sample in the western blot shown on the datasheet?

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    Answer

    The 48 kDa band on the datasheet western blot is a recombinant fragment, not the full-length protein. The laboratory that provided the data did not try to detect endogenous FAT4 by western blotting, since it is so large, and blotting proteins that large is difficult. The antibody should be able to detect the full-length, 350+ kDa protein, however. It detected a protein that is presumed to be FAT4 on D3 stem cells stained by immunocytochemistry with this antibody.

    If you are having difficulty detecting the endogenous protein by a standard western blot protocol (you will need a low-percentage polyacrylamide gel, 4-6%, and a long transfer to the membrane in methanol-free transfer buffer), you might have better luck with our "blue native" electrophoresis protocol. The protocol refers to SDS-PAGE of large mitochondrial proteins but it will be applicable to FAT4 too. Here is a link.

    https://www.abcam.com/index.html?pageconfig=resource&rid=14626

    Read More

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