Key features and details
- Rabbit polyclonal to FAT/FAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FAT/FAT1 antibody
See all FAT/FAT1 primary antibodies
DescriptionRabbit polyclonal to FAT/FAT1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human FAT/FAT1 aa 4484-4534. The exact sequence is proprietary. NP_005236.2
Database link: Q14517
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
This product was previously labelled as FAT
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to FAT/FAT1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
FunctionPlays an essential role for cellular polarization, directed cell migration and modulating cell-cell contact.
Tissue specificityExpressed in many epithelial and some endothelial and smooth muscle cells.
Sequence similaritiesContains 33 cadherin domains.
Contains 5 EGF-like domains.
Contains 1 laminin G-like domain.
DomainA PTB-like motif (DNXYH sequence) is required for the targeting to the leading edge. This motif represents a minimal protein-protein interaction core motif that is not regulated by tyrosine phosphorylation.
modificationsUndergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.
Cellular localizationCell membrane. Nucleus. Cytoplasm > perinuclear region.
- Information by UniProt
- Cadherin family member 7 antibody
- Cadherin family member 7 precursor antibody
- Cadherin ME5 antibody
All lanes : Anti-FAT/FAT1 antibody (ab241372) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241372 has not yet been referenced specifically in any publications.