Overview

  • Product name
    Anti-FAT/FAT1 antibody [Fat-1]
    See all FAT/FAT1 primary antibodies
  • Description
    Mouse monoclonal [Fat-1] to FAT/FAT1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
    Predicted to work with: Rat, Chimpanzee
  • Immunogen

    Other Immunogen Type corresponding to FAT/FAT1.

  • Positive control
    • Wild type imaginal discs from third instar larva
  • General notes

     This product was previously labelled as FAT

     

Properties

Applications

Our Abpromise guarantee covers the use of ab20163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 506 kDa.

Use 3-8% gradient gels to visualise the FAT/FAT1 protein and wet transfer.

ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Plays an essential role for cellular polarization, directed cell migration and modulating cell-cell contact.
  • Tissue specificity
    Expressed in many epithelial and some endothelial and smooth muscle cells.
  • Sequence similarities
    Contains 33 cadherin domains.
    Contains 5 EGF-like domains.
    Contains 1 laminin G-like domain.
  • Domain
    A PTB-like motif (DNXYH sequence) is required for the targeting to the leading edge. This motif represents a minimal protein-protein interaction core motif that is not regulated by tyrosine phosphorylation.
  • Post-translational
    modifications
    Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.
  • Cellular localization
    Cell membrane. Nucleus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin family member 7 antibody
    • Cadherin family member 7 precursor antibody
    • Cadherin ME5 antibody
    • Cadherin related tumor suppressor antibody
    • Cadherin related tumor suppressor homolog antibody
    • Cadherin related tumor suppressor homolog precursor antibody
    • Cadherin related tumor suppressor homolog precursor (FAT protein homolog) antibody
    • Cadherin-related tumor suppressor homolog antibody
    • CDHF 7 antibody
    • CDHF7 antibody
    • FAT 1 antibody
    • FAT tumor suppressor 1 antibody
    • FAT tumor suppressor homolog 1 antibody
    • FAT1 antibody
    • FAT1_HUMAN antibody
    • hFat 1 antibody
    • hFAT antibody
    • hFat1 antibody
    • Homolog of Drosophila FAT protein antibody
    • Homolog of Drosophila FAT protein precursor antibody
    • Homolog of Drosophila tumor suppressor FAT precursor antibody
    • ME 5 antibody
    • ME5 antibody
    • nuclear form antibody
    • Protein fat homolog antibody
    • Protocadherin Fat 1 antibody
    see all

References

This product has been referenced in:
  • Huang JW  et al. Systematic screen identifies miRNAs that target RAD51 and RAD51D to enhance chemosensitivity. Mol Cancer Res 11:1564-73 (2013). Read more (PubMed: 24088786) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. This product has not been tested in human. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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