Overview

  • Product name

  • Description

    Rabbit polyclonal to FATP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region within the N-terminal sequence 71-120 (LLFRDETLTY AQVDRRSNQV ARALHDHLGL RQGDCVALLM GNEPAYVWLW) of Human SLC27A2 (NP_003636).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab83763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31, 65, 90 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes.
  • Tissue specificity

    Expressed in liver, kidney, placenta and pancreas.
  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization

    Endoplasmic reticulum membrane. Peroxisome membrane. Peripheral membrane associated with the lumenal side of peroxisomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACSVL1 antibody
    • FACVL1 antibody
    • FATP 2 antibody
    • FATP-2 antibody
    • FATP2 antibody
    • Fatty acid coenzyme A ligase, very long chain 1 antibody
    • Fatty acid transport protein 2 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • hFACVL1 antibody
    • HsT17226 antibody
    • Long chain fatty acid CoA ligase antibody
    • Long-chain-fatty-acid--CoA ligase antibody
    • S27A2_HUMAN antibody
    • Slc27a2 antibody
    • Solute carrier family 27 (fatty acid transporter), member 2 antibody
    • Solute carrier family 27 member 2 antibody
    • THCA CoA ligase antibody
    • THCA-CoA ligase antibody
    • Very long chain acyl CoA synthetase antibody
    • Very long chain fatty acid CoA ligase antibody
    • Very long chain fatty acid coenzyme A ligase 1 antibody
    • very long-chain 1 antibody
    • Very long-chain acyl-CoA synthetase antibody
    • Very long-chain-fatty-acid-CoA ligase antibody
    • VLACS antibody
    • VLCS antibody
    see all

Images

  • Western blot analysis of HeLa cell lysate labeling FATP2 with ab83763 at 1.0µg/ml.
  • Western blot analysis of 293T cell lysate labeling FATP2 with ab83763 at 1.0µg/ml.
  • Anti-FATP2 antibody (ab83763) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 70 kDa
    Observed band size: 31,65,90 kDa
    why is the actual band size different from the predicted?



    Gel concentration 12%
  • All lanes : Anti-FATP2 antibody (ab83763) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate with blocking peptide at 5 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FATP2 with ab83763.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling FATP2 with ab83763 at 10µg/ml.

References

This product has been referenced in:

  • Martin GG  et al. Fabp1 gene ablation inhibits high-fat diet-induced increase in brain endocannabinoids. J Neurochem 140:294-306 (2017). Read more (PubMed: 27861894) »
  • Milligan S  et al. Impact of dietary phytol on lipid metabolism in SCP2/SCPX/L-FABP null mice. Biochim Biophys Acta Mol Cell Biol Lipids 1862:291-304 (2017). Read more (PubMed: 27940000) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Placenta)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Verified customer

Submitted Sep 14 2015

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