Overview

  • Product name

    Fatty Acid Oxidation Assay Kit (flow cytometry)
    See all Fatty Acid Oxidation kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Product overview

    Fatty Acid Oxidation Assay Kit ab118183 contains antibodies against key enzymes of the mitochondrial fatty acid oxidation pathway for flow cytometry. The assay combines the power of single cell analysis obtained from flow cytometry with the specificity of antibody-based immunostaining to quantify protein levels in cultured cells. Cells are harvested and fixed/permeabilized in suspension, targets of interest are detected indirectly with highly specific well-characterized monoclonal antibodies that are then labeled with fluorescent antibodies


     


    This product was previously called Fatty Acid Oxidation Human Flow Cytometry Kit.

  • Notes

    Spin down the contents of the antibody vials upon receipt of the kit. Store all components upright at 4°C. This kit is stable for at least 6 months from receipt.

  • Platform

    Flow cytometer

Properties

Images

  • Expression levels of ACADVL was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADVL:p[N122D]. Black: Background fluorescence was determined with the supplied negative control.

  • After background subtraction, the ACADVL deficient cell line shows a 93% reduction in the level of the ACADVL protein.

  • Expression levels of HADHA was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes HADHB:p[R61H];[R247H]. Black: Background fluorescence was determined with the supplied negative control.

  • After background subtraction, the LCHAD deficient cell line shows a 50% reduction in the level of the HADHA protein.

  • Expression levels of ACADM was determined in primary fibroblasts by flow cytometry. Blue: control cell line. Red: deficient cell lines extracted from patients with well characterized mutations in each of the enzymes ACADM:p[K604E] Black: Background fluorescence was determined with the supplied negative control.

  • After background subtraction, the ACADM deficient cell line shows a 57% reduction in the level of the ACADM protein.

  • Antibody specificity demonstrated by immunocytochemistry. Cells were processes with the Flow cytometry protocol as explained above. Panel A shows control fibroblasts and panel B shows deficient fibroblasts. Left panel shows staining with anti-ACADVL ab, center panel with anti-ACADM ab and right panel with anti-HADHA ab.

Protocols

References

This product has been referenced in:

  • Pascual G  et al. Targeting metastasis-initiating cells through the fatty acid receptor CD36. Nature 541:41-45 (2017). Read more (PubMed: 27974793) »
  • Tabe Y  et al. Bone Marrow Adipocytes Facilitate Fatty Acid Oxidation Activating AMPK and a Transcriptional Network Supporting Survival of Acute Monocytic Leukemia Cells. Cancer Res 77:1453-1464 (2017). Read more (PubMed: 28108519) »
See all 3 Publications for this product

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