Overview

  • Product name
    Anti-Fatty Acid Synthase antibody
    See all Fatty Acid Synthase primary antibodies
  • Description
    Rabbit polyclonal to Fatty Acid Synthase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC (PFA fixed), IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2450 to the C-terminus of Mouse Fatty Acid Synthase.

    Read Abcam's proprietary immunogen policy (Peptide available as ab25719.)

  • Positive control
    • WB: 3T3-L1 nuclear lysate (ab14632), mouse brain (ab27253), mouse liver whole cell lysate (ab7935), A549, HAP1 and human liver lysates. ICC/IF: HEK293 cells.

Applications

Our Abpromise guarantee covers the use of ab22759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).
IHC-P Use at an assay dependent concentration.
IHC (PFA fixed) Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 21098489

Target

  • Function
    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • Tissue specificity
    Ubiquitous. Prominent expression in brain, lung, and liver.
  • Sequence similarities
    Contains 1 acyl carrier domain.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab22759 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab22759 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab22759 and ab18058 (loading control to Vinculin) were diluted at 1 µg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ab22759 staining Fatty Acid Synthase in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in Citra Plus solution. Samples were incubated with primary antibody (2μg/ml) for 14 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab22759 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab22759, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions.

    The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Fatty Acid Synthase antibody (ab22759) at 1 µg/ml

    Lane 1 : 3T3-L1 nuclear extract lysate (ab14632)
    Lane 2 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 3 : Liver (Mouse) Tissue Lysate (ab7935)
    Lane 4 : 3T3-L1 nuclear extract lysate (ab14632) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 5 : Brain (Mouse) Tissue Lysate (ab27253) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml
    Lane 6 : Liver (Mouse) Tissue Lysate (ab7935) with Mouse Fatty Acid Synthase peptide (ab25719) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 273 kDa
    Observed band size: 273 kDa
    Additional bands at: 100 kDa, 35 kDa, 50 kDa (possible IgG). We are unsure as to the identity of these extra bands.

  • Immunofluorescent staining for Fatty Acid Synthase in the rat striatum using Rabbit polyclonal to Fatty Acid Synthase (ab22759). Abundant staining was observed in the Striatum with lower levels of staining observed in the Corpus callosum. This is a montage of three pictures aquired using a X10 objective. ab22759 was used at 1/200 (2µg/ml) incubated overnight at room temperature.

    Secondary antibody used was anti-rabbit Alexa Fluor® 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

References

This product has been referenced in:
  • Zhao X  et al. Osthole inhibits oleic acid/lipopolysaccharide-induced lipid accumulation and inflammatory response through activating PPARa signaling pathway in cultured hepatocytes. Exp Gerontol 119:7-13 (2019). Read more (PubMed: 30659956) »
  • Hu S  et al. Prenatal caffeine exposure increases the susceptibility to non-alcoholic fatty liver disease in female offspring rats via activation of GR-C/EBPa-SIRT1 pathway. Toxicology 417:23-34 (2019). Read more (PubMed: 30776459) »
See all 40 Publications for this product

Customer reviews and Q&As

11-16 of 16 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
40 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 02 2011

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Liver)
Total protein in input
1000 µg
Specification
Liver
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Feb 24 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hepa1-6 (murine hepatoma))
Specification
Hepa1-6 (murine hepatoma)
Fixative
10% formalin
Permeabilization
Yes - 0.1% Triton X100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 24 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Sections of rat brain)
Specification
Sections of rat brain
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Dec 10 2007

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary gland)
Specification
mammary gland
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mrs. Elise Bales

Verified customer

Submitted Apr 03 2007

Question
Answer

Thank you for contacting us.

I have included a pdf of the publication referenced on our datasheet which has used ab22759 in IP. I will also add the direct link below:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024788/

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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11-16 of 16 Abreviews or Q&A

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